文献类型： 外文期刊
作者： Yan, Huijun 1 ; Zhang, Zhao 2 ; Magnard, Jean-Louis 3 ; Boachon, Benoit 3 ; Baudino, Sylvie 3 ; Tang, Kaixue 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming, Yunnan, Peoples R China
2.China Agr Univ, Dept Ornamental Hort, Beijing Key Lab Dev & Qual Control Ornamental Cro, Beijing, Peoples R China
3.Univ Lyon, UJM St Etienne, CNRS, BVpam FRE 3727, St Etienne, France
关键词： VIGS; Rose; Vacuum infiltration; Graft; Posttranscriptional gene silencing
期刊名称：VIRUS-INDUCED GENE SILENCING IN PLANTS: Methods and Protocols
ISSN： 1064-3745
年卷期： 2020 年 2172 卷
页码：
收录情况： SCI
摘要： Virus-induced gene silencing (VIGS) is a favorable method to study gene function by posttranscriptional gene silencing in plants. Here we describe a methodology of graft-accelerated VIGS in rose aimed at obtaining posttranscriptional gene silencing in the flower. The resulting phenotype can be observed within 5-6 weeks post infiltration. By using this method, we successfully silenced the expression of several genes involved in processes such as scent production, petal coloration, or flower architecture. We showed that graft-accelerated VIGS was faster, more efficient, and more convenient than conventional methods previously developed in rose such as agroinfiltration of young plantlets and in vitro cultured tissues or seeds.
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