您好，欢迎访问云南省农业科学院 机构知识库！

In vitro propagation and long-term observation of acclimated plants in endangered tree fern Alsophila costularis

文献类型： 外文期刊

作者： Pu, Yanfei 2 ; Song, Qixuan 2 ; Wang, Guoxian 1 ; Wu, Lifang 1 ; Yang, Chunmei 1 ; Yu, Rongpei 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

2.Yunnan Univ, Resource Plant Res Inst, Kunming 650504, Yunnan, Peoples R China

3.Yuxi Yunxing Biotech Co Ltd, Yuxi 653100, Yunnan, Peoples R China

关键词： Tree fern; Green globular bodies; Cytokinin; Plant acclimatization

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

年卷期：

页码：

收录情况： SCI

摘要： Alsophila costularis Barker (Cyathea costularis), an endangered tree fern with tree-like erect stem, attracts gardening enthusiasts as a special ornamental plant. In vitro propagation can be advantageous for germplasm conservation and commercial application of A. costularis. Here, we described in vitro propagation of A. costularis via spore culture and green globular bodies (GGBs) system, as well as the long-term observation of acclimated plants regenerated from GGBs. In spore culture, the low concentration of mineral salt (1/8 MS) was beneficial for sporophyte formation on gametophytes, but sporophytes per conical flask was only 8 plantlets. In GGB system, cytokinin thidiazuron (TDZ) was essential for GGB induction and multiplication. The maximum of GGB induction frequency (93.33%) was obtained on 1/2MS medium with 2.0 mg/l TDZ by using juvenile sporophytes as explants, and the same medium was optimal for GGB multiplication. 1/4 MS supplemented with 0.1% (w/v) activated carbon (AC) was appropriate for plantlet regeneration from GGB, GGB differentiation frequency was 100%, and 42.40 plantlets could be regenerated from one piece of GGBs. The maximum of plantlet height (4.64 cm) was obtained on 1/2 MS with 0.1% (w/v) AC. After 6 years of acclimatization cultivation for plantlets regenerated from GGBs, plants in plastic pots with diameter of 60 cm showed an excellent vegetative and reproductive growth, and the mature spores of these plants could produce sporophytes. Morphological and histological observation demonstrated that A. costularis GGBs was a green structure that consisted of multiple single GGBs with hair-like structures. One single GGB could develop into one plantlet. Key message Establishment of an in vitro propagation protocol of endangered tree fern Alsophila costularis, and long-term observation of acclimated plants regenerated from green globular bodies (GGBs) in A. costularis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台