Transcriptomic and proteomic analyses of Cucurbita ficifolia Bouche (Cucurbitaceae) response to Fusarium oxysporum f.sp. cucumerium
文献类型： 外文期刊
作者： Xie, Junjun 1 ; Ding, Yumei 2 ; Gao, Ting 1 ; He, Shuilian 1 ; Zhao, Kai 1 ; Yang, Xuehu 1 ; Zhang, Jie 1 ; Yang, Zhengan 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China
3.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650205, Yunnan, Peoples R China
关键词： Cucurbita ficifolia Bouche; FOC; iTRAQ; RNA-sequencing; Defense response
期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）
ISSN： 1471-2164
年卷期： 2022 年 23 卷 SUPPL 1 期
页码：
收录情况： SCI
摘要： Background Fusarium oxysporum f. sp. cucumerinum (FOC) is the causal agent of cucumber Fusarium wilt, which can cause extensive damages and productivity losses. Cucurbita ficifolia Bouche (Cucurbitaceae) is usually used as rootstock for cucumber because of its excellent resistance to Fusarium wilt. Our previous study found that C.ficifolia has high FOC resistance, the underlying mechanism of which is unclear. Results Transcriptome and proteome profiling was performed on the basis of RNA-Seq and isobaric tag for relative and absolute quantitation technology to explore the molecular mechanisms of the response of Cucurbita ficifolia Bouche to Fusarium oxysporum f. sp. cucumerium infection. Comparative analyses revealed that 1850 genes and 356 protein species were differentially regulated at 2d and 4d after FOC inoculation. However, correlation analysis revealed that only 11 and 39 genes were differentially regulated at both the transcriptome and proteome levels after FOC inoculation at 2d and 4d, respectively. After FOC inoculation, plant hormones signal transduction, transcription factors were stimulated, whereas wax biosynthesis and photosynthesis were suppressed. Increased synthesis of oxidative-redox proteins is involved in resistance to FOC. Conclusions This study is the first to reveal the response of C. ficifolia leaf to FOC infection at the transcriptome and proteome levels, and to show that FOC infection activates plant hormone signaling and transcription factors while suppressing wax biosynthesis and photosynthesis. The accumulation of oxidative-redox proteins also plays an important role in the resistance of C. ficifolia to FOC. Results provide new information regarding the processes of C. ficifolia leaf resistance to FOC and will contribute to the breeding of cucumber rootstock with FOC resistance.
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