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Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily

文献类型： 外文期刊

作者： Sui, Juanjuan 1 ; Jia, Wenjie 2 ; Xin, Yin 2 ; Zhang, Yuanyuan 4 ;

作者机构： 1.Fuyang Normal Univ, Biol & Food Engn Coll, Dept Biol, Fuyang 236037, Peoples R China

2.China Agr Univ, Dept Ornamental Hort & Landscape Architecture, Beijing Key Lab Dev & Qual Control Ornamental Cro, Beijing 100193, Peoples R China

3.Yunnan Acad Agr Sci, Flower Res Inst, Kunming 650205, Yunnan, Peoples R China

4.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

关键词： lily; pollen grains; tapetum degradation; microspore development; transcriptome

期刊名称：GENES （ 影响因子：4.141； 五年影响因子：4.474 ）

ISSN：

年卷期： 2022 年 13 卷 2 期

页码：

收录情况： SCI

摘要： Lily is a popular and economically ornamental crop around the world. However, its high production of pollen grains causes serious problems to consumers, including allergies and staining of clothes. During anther development, the tapetum is a crucial step for pollen formation and microspore release. Therefore, it is important to understand the mechanism of tapetum degradation and microspore development in lily where free pollen contamination occurs. Here, we used the cut lily cultivar 'Siberia' to characterize the process of tapetum degradation through the use of cytology and transcriptomic methods. The cytological observation indicated that, as the lily buds developed from 4 cm (Lo 4 cm) to 8 cm (Lo 8 cm), the tapetum completed the degradation process and the microspores matured. Furthermore, by comparing the transcriptome profiling among three developmental stages (Lo 4 cm, Lo 6 cm and Lo 8 cm), we identified 27 differentially expressed genes. These 27 genes were classed into 4 groups by function, namely, cell division and expansion, cell-wall morphogenesis, transcription factors, LRR-RLK (leucine-rich repeat receptor-like kinases), plant hormone biosynthesis and transduction. Quantitative real-time PCR was performed as validation of the transcriptome data. These selected genes are candidate genes for the tapetum degradation and microspore development of lily and our work provides a theoretical basis for breeding new lily cultivars without pollen.

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