Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco
文献类型： 外文期刊
作者： Ma, Lulin 1 ; Jia, Wenjie 1 ; Duan, Qing 1 ; Du, Wenwen 1 ; Li, Xiang 1 ; Cui, Guangfen 1 ; Wang, Xiangning 1 ; Wang, Jihua 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Key Lab Yunnan Flower Breeding, Flower Res Inst, Kunming 650205, Peoples R China
关键词： PgF3'5'H; transgenic tobacco; over-expression; delphinidin-based anthocyanin; blue flower color
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2023 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Flavonoid-3 ',5 '-hydroxylase (F3 ' 5 ' H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3 ' 5 ' H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3 ' 5 ' H. In this study, PgF3 ' 5 ' H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T-0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3 ' 5 ' H showed a higher expression level in all PgF3 ' 5 ' H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3 ' 5 ' H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3 ' 5 ' H transgenic tobacco plants by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3 ' 5 ' H products have F3 ' 5 ' H enzyme activity. In addition, PgF3 ' 5 ' H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3 ' 5 ' H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.
- 相关文献
作者其他论文 更多>>
-
Transcriptomic Profiling of Heat-Treated Oriental Lily Reveals LhERF109 as a Positive Regulator of Anthocyanin Accumulation
作者：Zhou, Mei;Zeng, Lijia;Zhu, Jungang;Yong, Xue;Wu, Mengxi;Jiang, Beibei;Jia, Yin;Li, Fan;Jin, Chunlian;Wang, Jihua;Li, Fan;Jin, Chunlian;Yuan, Huijuan;Wang, Jihua;Yuan, Huijuan;Pan, Yuanzhi
关键词：oriental lily; heat stress; transcriptome; anthocyanin biosynthesis; WGCNA;
LhERF109 -
The giant genome of lily provides insights into the hybridization of cultivated lilies
作者：Liang, Yuwei;Gao, Qiang;Zhang, Liangsheng;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Du, Yunpeng;Pan, Wenqiang;Zhang, Xiuhai;Zhang, Mingfang;Wu, Jian;Pan, Wenqiang;Wang, Shaokun;Song, Xiaoming;Zhong, Linlin;Zhang, Fan;Li, Yan;Wang, Zhiwei;Li, Danqing;Gu, Yang;Chen, Zhong-Hua;Chen, Zhong-Hua;Mayer, Klaus F. X.;Mayer, Klaus F. X.;Zhou, Xiaofan;Zhang, Liangsheng;Zhang, Liangsheng
关键词：
-
Anthocyanin biosynthesis and transport synergistically modulated by RcMYB75 and RcGSTFL11 play a pivotal role in the feedforward loop in response to drought stress
作者：Ma, Mengni;Li, Runhui;Li, Yajun;Dai, Wenhao;Shang, Junzhong;He, Yanhong;Zhang, Jie;Ning, Guogui;Ma, Mengni;Li, Runhui;Li, Yajun;Dai, Wenhao;Shang, Junzhong;He, Yanhong;Zhang, Jie;Ning, Guogui;Xiang, Fayun;Yang, Yuanyuan;Wang, Jihua;Huang, Zifeng;Luo, Hong
关键词：MYB transcription factor; glutathione S-transferases; anthocyanin; drought;
Rosa chinensis -
Herbicide Screening and Application Method Development for Sustainable Weed Management in Tagetes erecta L. Fields
作者：Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Feng, Dongyan;Jia, Chengcheng;Tang, Dongsheng;Huang, Junhong;Li, Ye
关键词：weed management; pre-emergence herbicides; post-emergence herbicides; crop safety
-
The circadian clock module LgPRR7-LgFKF1 negatively regulates flowering time in Luculia gratissima, a woody ornamental plant
作者：Liu, Xiongfang;Wan, Youming;Li, Zhenghong;Zhang, Yao;Ma, Hong;Liu, Xiongfang;Gao, Fu;Ma, Yongpeng;Wang, Jihua;Wang, Jihua;Wu, Zihuan;Ma, Hong;Ma, Hong
关键词：
-
A special short-wing petal faba genome and genetic dissection of floral and yield-related traits accelerate breeding and improvement of faba bean
作者：Liu, Rong;Li, Mengwei;Shu, Qin;Wang, Zonghe;Zong, Xuxiao;Yang, Tao;Hu, Chaoqin;Yang, Xin;Dai, Zhengming;Yu, Haitian;Yang, Feng;Zheng, Aiqing;Lv, Meiyuan;He, Yuhua;Gao, Dan;Chen, Liyang;Jiao, Chengzhi;Yuan, Xingxing;Chen, Xin;Garg, Vanika;Chitikineni, Annapurna;Barmukh, Rutwik;Varshney, Rajeev K.;Zhang, Hongyan;Hou, Wanwei;Teng, Changcai;Zhou, Xianli;Liu, Yujiao;Zhang, Hongyan;Hou, Wanwei;Teng, Changcai;Zhou, Xianli;Du, Chengzhang;Xiang, Chao;Xu, Dongxu;Tang, Yongsheng;Duan, Yinmei;Maalouf, Fouad;Agrawal, Shiv Kumar;Wei, Libin;Zhao, Na;Li, Xiang;Wang, Dong;Ding, Hanfeng
关键词：Faba bean; Short-wing petal; Genome assembly; Genomic diversity; GWAS; Yield-related loci; Breeding; Improvement
-
Nine complete chloroplast genomes of the Camellia genus provide insights into evolutionary relationships and species differentiation
作者：Cai, Yanfei;Tian, Min;Yang, Yingjie;Shi, Ziming;Zhao, Peifei;Wang, Jihua;Cai, Yanfei;Tian, Min;Yang, Yingjie;Shi, Ziming;Zhao, Peifei;Wang, Jihua
关键词：Camellia genus; Camellia japonica; Chloroplast genome; Genome structure; SSR molecular marker; Phylogenetic analysis
