Fine Mapping of Clubroot Resistance Loci CRA8.1 and Candidate Gene Analysis in Chinese Cabbage (Brassica rapa L.)
文献类型: 外文期刊
作者: Wang, Yanyan 1 ; Xiang, Xianyu 1 ; Huang, Fan 1 ; Yu, Wenlin 1 ; Zhou, Xueqing 1 ; Li, Baojun 1 ; Zhang, Yunyun 1 ; Chen, Peng 1 ; Zhang, Chunyu 1 ;
作者机构: 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China
2.Hybrid Rape Res Ctr Shaanxi Prov, Shaanxi Rapeseed Branch Natl Ctr Oil Crops Genet I, Yangling, Peoples R China
3.Ind Crops Res Inst, Yunnan Acad Agr Sci, Kunming, Peoples R China
关键词: clubroot; Plasmodiophora brassicae; Brassica rapa; CRA8; 1; fine mapping
期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:6.627; 五年影响因子:7.255 )
ISSN: 1664-462X
年卷期: 2022 年 13 卷
页码:
收录情况: SCI
摘要: Clubroot is caused by Plasmodiophora brassicae, which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with different resistant capabilities from Huashuang5R (H5R) and Huayouza62R of Brassica napus, which are currently used as the main resistant cultivars for clubroot management in China. We used a next-generation sequencing-based bulked segregant analysis approach, combined with genetic mapping to identify clubroot-resistant (CR) genes from F-1 population generated from a cross between the DW (CR) and HZSX (clubroot susceptible). The CR locus of DW (named CRA8.1) was mapped to a region between markers A08-4346 and A08-4853, which contains two different loci CRA8.1a and CRA8.1b after fine mapping. The CRA8.1b loci contain a fragment of 395 kb between markers A08-4624 and A08-4853 on A08 chromosome, and it is responsible for the resistance to PbZj and PbXm isolates. However, together with CRA8.1a, corresponding to a 765-kb region between markers A08-4346 and A08-4624, then it can confer resistance to PbXm(+). Finally, through expression analysis between resistant and susceptible materials, two genes encoding TIR-NBS-LRR proteins (BraA08g039211E and BraA08g039212E) and one gene encoding an RLP protein (BraA08g039193E) were identified to be the most likely CR candidates for the peculiar resistance in DW.
- 相关文献
作者其他论文 更多>>
-
Development of a rapid and efficient system for CR genes identification based on hairy root transformation in Brassicaceae
作者:Yu, Wenlin;Yang, Lu;Xiang, Yuanyuan;Zhou, Xueqing;Gan, Longcai;Xiang, Xianyu;Chen, Peng;Zhang, Chunyu;Yu, Wenlin;Yang, Lu;Xiang, Yuanyuan;Zhou, Xueqing;Gan, Longcai;Xiang, Xianyu;Chen, Peng;Zhang, Chunyu;Chen, Yinhua;Li, Rongde;Zhang, Yunyun;Yuan, Lei;Luo, Yanqing;Li, Genze;Wang, Youning
关键词:Brassicaceae; Agrobacterium rhizogenes; Hairy root transformation; Clubroot; Gene function
-
Pangenome analysis provides insight into the evolution of the orange subfamily and a key gene for citric acid accumulation in citrus fruits
作者:Huang, Yue;He, Jiaxian;Xu, Yuantao;Zheng, Weikang;Jiang, Xiaolin;Liu, Zishuang;Wang, Lun;Wang, Xia;Liu, Shengjun;Lu, Zhihao;Liu, Ziang;Yu, Huiwen;Xie, Zongzhou;Li, Chunlong;Ma, Zhaocheng;Jiao, Wenbiao;Zhang, Fei;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Huang, Yue;Xu, Yuantao;Xu, Qiang;Wang, Shaohua;Yue, Jianqiang;Gao, Junyan;Zhou, Xianyan;Long, Chunrui;Chen, Peng;Zeng, Bin;Yang, Shuizhi;Zeng, Xiuli;Guo, Yong-Jie;Guo, Yong-Jie;Zhang, Wen-Fu;Krueger, Robert R.;Smith, Malcolm W.;Ming, Ray
关键词:
-
Nitric oxide is involved in the regulation of guard mother cell division by inhibiting the synthesis of ACC
作者:Zhou, Lijuan;Yu, Shuangshuang;Liu, Yue;Wen, Yuanyuan;Zhang, Zijing;Ru, Yanyu;He, Zhaorong;Chen, Xiaolan;Zhou, Lijuan;Wang, Yanyan
关键词:ACS gene; GMC division; HB1; SGC; stoma
-
Whole-Genome Resequencing Reveals the Genetic Diversity and Selection Signatures of the Brassica juncea from the Yunnan-Guizhou Plateau
作者:Yuan, Xiaoyan;Liu, Hao;Li, Xuan;He, Liu;Liu, Feihu;Yuan, Xiaoyan;Fu, Minglian;Li, Genze;Zhang, Yunyun;Zhang, Yusong;Zhao, Kaiqin;Zhang, Lifan;Luo, Yanqing;Li, Jinfeng;He, Xiaoying;Qu, Cunmin
关键词:Brassica juncea; GWAS; seed coat color; fatty acid
-
Identification of Micro Ribonucleic Acids and Their Targets in Response to Plasmodiophora brassicae Infection in Brassica napus
作者:Li, Qian;Shah, Nadil;Zhou, Xueqing;Wang, Huiying;Yu, Wenlin;Liu, Chao;Zhang, Chunyu;Chen, Peng;Luo, Jiajie;Liu, Yajun;Li, Genze
关键词:Plasmodiophora brassicae; clubroot resistance; miRNA; degradome; Brassica napus
-
Hydrogen sulfide acts downstream of jasmonic acid to inhibit stomatal development in Arabidopsis
作者:Deng, Guobin;Zhou, Lijuan;Wang, Yanyan;Zhang, Gensong;Chen, Xiaolan;Wang, Yanyan
关键词:Hydrogen sulfide; Jasmonates; l-cysteine desulfhydrase; SPEECHLESS; Stomatal development