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Molecular analysis of five rice blast resistant genes in landraces from Myanmar and Laos

文献类型： 外文期刊

作者： Ma, Jiqiong 1 ; Sun, Yiding 1 ; Yang, Yi 1 ; Tang, Cuifeng 1 ; A, Xinxiang 1 ; Xu, Minghui 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Inst Biotechnol & Genet Resources, Sci Observat Stn Rice Germplasm Resources Yunnan, Minist Agr,Key Lab Biotechnol Res Yunnan Prov,Key, Kunming 650205, Yunnan, Peoples R China

关键词： blast resistance; DNA marker; landrace; Magnaporthe grisea; Oryza sativa; sequencing

期刊名称：PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION （ 影响因子：1.08； 五年影响因子：1.243 ）

ISSN： 1479-2621

年卷期： 2020 年 18 卷 4 期

页码：

收录情况： SCI

摘要： In order to understand rice blast resistance gene patterns in rice landraces from Myanmar and Laos, we analysed the Pita, Pib, Pikh, Pi9 and Pi5 genes in 80 landraces through the use of functional molecular markers and functional fragment sequencing. These landraces were separated into four haplotypes (H1-H4) based upon three mutations identified in the Pita sequence. All four haplotypes were detected among landraces from Myanmar, whereas only the H1 and H2 haplotypes were detected among landraces from Laos. We additionally grouped landraces carrying 0-4 resistance genes into 18 genotypes based upon their resistance-susceptibility patterns and found that 16 of these genotypes were detected among Myanmar landraces at relative frequencies ranging from 2.50-12.50%, while 10 were detected among landraces from Laos at relative frequencies of 2.50-42.50%. The 'Pib( + ) pikh(-) pi9(-) pi5(-) pita(-)' genotype was found to be dominant, accounting for 12.5 and 42.5% of landraces from Myanmar and Laos, respectively. The Pib, Pikh, Pi5 and Pita genes were detected in 52.50, 32.50, 37.50 and 30.00% of landraces from Myanmar respectively, whereas they were detected in 52.50, 12.50, 30.00 and 17.50% of landraces from Laos, respectively. The Pi9 gene was rarely detected among analysed landraces and was particularly rare in those from Laos. These findings suggest that rice landraces from Myanmar are more genetically diverse than those from Laos, with clear differences in blast resistance gene patterns between landraces from these two countries.

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