文献类型： 外文期刊

作者： Li, Ping ¹ ; Long, Junru ¹ ; Bai, Genxiang ¹ ; Zhang, Jian ¹ ; Cha, Yunsheng ² ; Gao, Wenjie ¹ ; Luan, Xinbo ¹ ; Wu, Lianzhang ² ; Mu, Mingxing ² ; Kennelly, Edward J. ³ ; Gao, Penghui ² ; Liu, Yuanyuan ⁵ ; Sun, Lirong ⁹ ; Yang, Quan ⁶ ; Wang, Guanhua ⁷ ; Yu, Zhijian ⁸ ; He, Juncai ² ; Yang, Yi ² ; Yan, Jian ¹ ;

作者机构： 1.South China Agr Univ, Coll Nat Resources & Environm, Guangdong Engn Res Ctr Modern Ecoagr, Key Lab Agroenvironm Trop,Minist Agr & Rural Affai, Guangzhou 510642, Peoples R China

2.Nujiang Green Spice Ind Res Inst, Lushui 673100, Yunnan, Peoples R China

3.CUNY, Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA

4.CUNY, Grad Ctr, Bronx, NY 10468 USA

5.Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Biotechnol & Germplasm Resources Inst, Key Lab Southwestern Crop Gene Resources & Germpla, Kunming 650205, Yunnan, Peoples R China

6.Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Guangzhou 510006, Peoples R China

7.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

8.Nujiang State Meteorol Bur, Lushui 673199, Yunnan, Peoples R China

9.Southern Med Univ, Sch Basic Med Sci, Dept Neurobiol, State Key Lab Organ Failure Res,Key Lab Mental Hlt, Guangzhou 510515, Peoples R China

关键词： Amomum tsao-ko; metabolomics; transcriptomics; biosynthetic pathways; diarylheptanoids

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 18 期

页码：

收录情况： SCI

摘要： Amomum tsao-ko is an important spice and medicinal plant that has received extensive attention in recent years for its high content of bioactive constituents with the potential for food additives and drug development. Diarylheptanoids are major and characteristic compounds in A. tsao-ko; however, the biochemical and molecular foundation of diarylheptanoids in fruit is unknown. We performed comparative metabolomics and transcriptomics studies in the ripening stages of A. tsao-ko fruit. The chemical constituents of fruit vary in different harvest periods, and the diarylheptanoids have a trend to decrease or increase with fruit development. GO enrichment analysis revealed that plant hormone signaling pathways including the ethylene-activated signaling pathway, salicylic acid, jasmonic acid, abscisic acid, and response to hydrogen peroxide were associated with fruit ripening. The biosynthetic pathways including phenylpropanoid, flavonoids, and diarylheptanoids biosynthesis were displayed in high enrichment levels in ripening fruit. The molecular networking and phytochemistry investigation of A. tsao-ko fruit has isolated and identified 10 diarylheptanoids including three new compounds. The candidate genes related to diarylheptanoids were obtained by coexpression network analysis and phylogenetic analysis. Two key genes have been verified to biosynthesize linear diarylheptanoids. This integrative approach provides gene regulation and networking associated with the biosynthesis of characteristic diarylheptanoids, which can be used to improve the quality of A. tsao-ko as food and medicine.