Pepper mild mottle virus can infect and traffick within Nicotiana benthamiana plants in non-virion forms
文献类型： 外文期刊
作者： Zheng, Kaiyue 1 ; Zhang, Ruihao 2 ; Wan, Qionglian 1 ; Zhang, Ge 1 ; Lu, Yuwen 1 ; Zheng, Hongying 1 ; Yan, Fei 1 ; Peng, Jiejun 1 ; Wu, Jian 1 ;
作者机构： 1.Ningbo Univ, Inst Plant Virol, Key Lab Biotechnol Plant Protect MARA & Zhejiang P, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
2.Yunnan Acad Agr Sci, Hort Res Inst, Kunming 650205, Yunnan, Peoples R China
3.Yuxi Normal Univ, Sch Chem Biol & Environm, Yuxi 653100, Peoples R China
关键词： Virus transport; Long-distance transport; Pepper mild mottle virus; Coat protein; Root infection; Non-virion form
期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）
ISSN： 0042-6822
年卷期： 2023 年 587 卷
页码：
收录情况： SCI
摘要： Virions are responsible for the long-distance transport of many viruses, such as Pepper mild mottle virus (PMMoV). Emerging evidence indicates viral traffic in the form of ribonucleoprotein complexes (RNP), yet comprehensive analysis is scarce. In this study, we inoculated plants with PMMoV-GFP, both with and without the coding sequence for the coat protein (CP). PMMoV-GFP was detected in systemic leaves, even in the absence of the CP, despite the presence of much smaller infection areas. Moreover, using leaf extracts from PMMoV-infected plants to perform a root-irrigation experiment, we confirmed that PMMoV can infect plants through root transmission. Diluting the leaf extracts significantly diminished infectivity, and attempts to compensate for the dilution of other components by adding virions above the original level proved ineffective. Our findings strongly indicate that PMMoV can infect and traffick within plants in non-virion forms. Future studies should aim to identify the specific forms involved.
- 相关文献
作者其他论文 更多>>
-
Dissection of Metabolome and Transcriptome-Insights into Capsaicin and Flavonoid Accumulation in Two Typical Yunnan Xiaomila Fruits
作者：Hu, Huaran;Du, Lei;Zhang, Ruihao;Zhong, Qiuyue;Liu, Fawan;Li, Weifen;Gui, Min
关键词：pepper; capsaicin; flavonoid; phenylpropanoid; metabolome; transcriptome; transcription factor
-
Adoption of the 2A Ribosomal Skip Principle to Track Assembled Virions of Pepper Mild Mottle Virus in Nicotiana benthamiana
作者：Jiao, Mengting;Tian, Yanzhen;Lei, Jianing;Lin, Lin;Wu, Jian;Lu, Yuwen;Zheng, Hongying;Yan, Fei;Peng, Jiejun;Jiao, Mengting;Tian, Yanzhen;Lei, Jianing;Lin, Lin;Wu, Jian;Lu, Yuwen;Zheng, Hongying;Yan, Fei;Peng, Jiejun;Jiao, Mengting;Wang, Jianguang;Yin, Yueyan
关键词：2A; pepper mild mottle virus; coat protein; self-assembly
-
Melatonin delays softening of postharvest pepper fruits (Capsicum annuum L.) by regulating cell wall degradation, membrane stability and antioxidant systems
作者：Li, Pingping;Zhang, Ruihao;Zhou, Huidan;Mo, Yunrong;Wu, Shuang;Zhang, Xiang;Xie, Zhihe;Zhang, Tianyu;Zhao, Kai;Lv, Junheng;Deng, Minghua;Li, Pingping
关键词：Green pepper Capsicum annuum L.; Melatonin; Postharvest storage; Cell wall degradation; Antioxidant system; Fruit softening
-
Pesticide exposure and forage shortage in rice cropping system prevents honey bee colony establishment
作者：Liu, Feng;Zhang, Chuanlian;Zhou, Weiliang;Xu, Xijian;Shou, Qinyi;Yuan, Fang;Li, Qian;Huang, Huijun;Hu, Jinghua;Jiang, Wujun;Qin, Jiamin;Ye, Wuguang;Liu, Feng;Dai, Pingli;Zhang, Ge;Qin, Jiamin
关键词：Forest; Insecticide; Pesticide; Floral resource; Apis mellifera; Colony health
-
Integrative Metabolome and Transcriptome Analyses Provide Insights into Carotenoid Variation in Different-Colored Peppers
作者：Lv, Junheng;Zhang, Ruihao;Mo, Yunrong;Zhou, Huidan;Li, Mengjuan;Wu, Rui;Cheng, Hong;Zhang, Mingxian;Wang, Huasu;Hua, Wei;Deng, Qiaoling;Zhao, Kai;Deng, Minghua;Zhang, Ruihao
关键词：pepper; carotenoid; color formation; metabolome; transcriptome
-
Complete genome sequence of Paris polyphylla chlorotic mottle virus infecting Paris polyphylla var. yunnanensis in southwest China
作者：He, Qiongji;Chen, Binghua;Chen, Binghua;Zheng, Hongying;Hua, Mengyin;Yin, Yueyan;Peng, Jiejun;Li, Junmin;Chen, Jianping;Yan, Fei;Lin, Lin;Chen, Binghua;Cao, Yuhao;Song, Xuemei
关键词：
-
Adaption of tobacco rattle virus to its solanaceous hosts is related to the codon usage bias of the hosts and that of the viral 16 K gene
作者：Lin, Qi;Chen, Yi;Li, Miaomiao;Zheng, Hongying;Chen, Jianping;Yan, Fei;Lu, Yuwen;Zheng, Yuanting;Xu, Shengchun;Yin, Yueyan
关键词：Tobacco rattle virus (TRV); Codon usage bias; Host adaptation; 16K
