Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A
文献类型： 外文期刊
作者： Song, Liping 1 ; Li, Xia 3 ; Zu, Feng 4 ; Gao, Changbin 1 ; Wang, Bincai 1 ; Lin, Chufa 1 ; Tu, Jinxing 2 ; Wang, Aihua 1 ;
作者机构： 1.Wuhan Acad Agr Sci & Technol, Wuhan Vegetable Res Inst, Wuhan 430345, Hubei, Peoples R China
2.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China
3.Yunnan Acad Agr Sci, Biotechnol & Genet Resources Inst, Kunming, Yunnan, Peoples R China
4.Yunnan Acad Agr Sci, Ind Crops Inst, Kunming, Yunnan, Peoples R China
关键词： Differentially expressed genes; Genic-male sterility; Non-heading Chinese cabbage
期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）
ISSN： 1976-9571
年卷期： 2019 年 41 卷 12 期
页码：
收录情况： SCI
摘要： Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage.
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