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Lignin degradation potential and draft genome sequence of Trametes trogii S0301

文献类型： 外文期刊

作者： Liu, Yuan 1 ; Wu, Yuanyuan 1 ; Zhang, Yu 1 ; Yang, Xulei 1 ; Yang, En 1 ; Xu, Huini 1 ; Yang, Qiliang 3 ; Chagan, Irbis 1 ;

作者机构： 1.Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming 650500, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Biotechnol & Germplasm Resources Inst, Kunming 650223, Yunnan, Peoples R China

3.Kunming Univ Sci & Technol, Fac Modern Agr Engn, Kunming 650500, Yunnan, Peoples R China

4.Yunnan Prov Key Lab Panax Notoginseng, Kunming 650500, Yunnan, Peoples R China

5.State Adm Tradit Chinese Med, Key Lab Panax Notoginseng Resources Sustainable D, Kunming 650500, Yunnan, Peoples R China

6.Kunming Key Lab Sustainable Dev & Utilizat Famous, Kunming 650500, Yunnan, Peoples R China

关键词： Trametes trogii; Genome sequencing; Lignin degradation; CAZymes; Laccases

期刊名称：BIOTECHNOLOGY FOR BIOFUELS （ 影响因子：6.04； 五年影响因子：6.485 ）

ISSN：

年卷期： 2019 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Background: Trametes trogii is a member of the white-rot fungi family, which has a unique ability to break down recalcitrant lignin polymers to CO2 and water, and they have enormous potential to biodegrade a wide range of toxic environmental pollutants. Because of its industrial potential, the identification of lignin-degrading enzyme systems in Trametes is an important area of research. Development and utilization of industrial value genes are suffering due to deficiency knowledge of genome available for their manipulation. Results: In the present study, Homokaryotic strains of T. trogii S0301 were screened and sequencing by PacBio Sequel II platform. The final draft genome is similar to 39.88 Mb, with a contig N50 size of 2.4 Mb, this was the first genome sequencing and assembly of T. trogii species. Further analyses predicted 14,508 protein-coding genes. Results showed that T. trogii S0301 contains 602 genes encoding CAZymes, include 211 glycoside hydrolase and 117 lignin-degrading family genes, nine laccases related genes. Small subunit ribosomal RNA gene (18S rRNA) sequencing confirms its phylogenetic position. Moreover, T. trogii S0301 has the largest number of cytochromes P450 (CYPs) superfamily genes compare to other fungi. All these results are consistent with enzymatic assays and transcriptome analysis results. We also analyzed other genome characteristics in the T. trogii S0301genome. Conclusion: Here, we present a nearly complete genome for T. trogii S0301, which will help elucidate the biosynthetic pathways of the lignin-degrading enzyme, advancing the discovery, characterization, and modification of novel enzymes from this genus. This genome sequence will provide a valuable reference for the investigation of lignin degradation in the Trametes genus.

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