您好，欢迎访问云南省农业科学院 机构知识库！

Analysis of genetic variations of heat shock proteins Hsp70 and Hsp90 in Isaria farinosa strains from the Yunnan province of China

文献类型： 外文期刊

作者： He, Lu 1 ; Yu, Hong 1 ; Dai, Yongdong 1 ; Wang, Yuanbing 1 ;

作者机构： 1.Yunnan Univ, Sch Life Sci, Inst Herb Biot Resources, Yunnan Herbal Lab, Kunming 650091, Yunnan, Peoples R China

2.Yunnan Acad Agr Sci, Inst Trop Ecoagr, Yuanmou 651399, Peoples R China

3.Yunnan Univ, Int Joint Res Ctr Sustainable Utilizat Cordyceps, Kunming 650091, Yunnan, Peoples R China

4.Yunnan Herbal Biotech Co Ltd, Res Ctr Cordyceps Dev & Utilizat Kunming, Kunming 650106, Yunnan, Peoples R China

期刊名称：FOLIA MICROBIOLOGICA （ 影响因子：2.099； 五年影响因子：2.131 ）

ISSN： 0015-5632

年卷期： 2019 年 64 卷 4 期

页码：

收录情况： SCI

摘要： In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populations distributed in the Yunnan province. The complete sequence of the IFHSP70 cDNA had a length of 2158bp, and contained an open reading frame (ORF) of 1962bp, encoding a 71-kDa polypeptide comprising of 653 amino acids. IFHSP90 cDNA had a length of 2144bp, and contained an ORF of 2103bp, encoding a polypeptide of 79.23kDa, comprising of 700 amino acids. The deduced amino acid sequences of IFHSP70 and IFHSP90 shared high sequence identities with other fungi. Fundamental information pertaining to the protein families, signatures, and conserved motifs of Hsp70 and Hsp90 were also identified. Analysis of molecular variances (AMOVA) from the Hsp70 and Hsp90 genes showed that the genetic variation within-population (83.26%, 83.08%) was greater than among the populations (16.74%, 16.92%). The values of nucleotide diversity (Pi), haplotype diversity (Hd), coefficient of genetic differentiation (Fst), and gene flow (Nm) were calculated. For Hsp70, Pi=0.0425, Hd=0.888, Fst=0.167, Nm=1.24; For Hsp90, Pi=0.0420, Hd=0.894, Fst=0.169, and Nm=1.22. These data indicated that the genetic differentiation among 10 different geographical populations of I. farinosa was limited. This study describes, for the first time, cloning, characterization and identification of Isaria farinosa Hsp70 and Hsp90 genes, and provides a preliminary basis for exploring the genetic structure of the genus Isaria using the sequences of Hsp70 and Hsp90 as molecular markers.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台