文献类型： 外文期刊

作者： Zhu, Xiuying ¹ ; Yan, Hegui ¹ ; Yang, Minghui ¹ ; Yang, Xiaoqiong ² ; He, Lu ² ;

作者机构： 1.Dali Univ, Coll Pharm, Dali 671000, Peoples R China

2.Yunnan Acad Agr Sci, Inst Trop Ecoagr Sci, Yuanmou 651300, Peoples R China

期刊名称：JOURNAL OF AOAC INTERNATIONAL （ 影响因子：2.028； 五年影响因子：2.008 ）

ISSN： 1060-3271

年卷期： 2022 年 105 卷 1 期

页码：

收录情况： SCI

摘要： Background Pholidota articulata Lindl has been used as a traditional medicine and Yi-nationality herbal medicine for the treatment of various diseases. Phenanthrenes and bibenzyls commonly found in the genus Pholidota are one of their most important natural ingredients, due to their various biological activities. Objective This study aimed to establish an HPLC method for determination of the levels of three phenenthrenes (flavidin, lusianthridin, coelonin) and two bibenzyls (batatasin III, cirrhopetalidin) in rhizomes of P. articulata Lindl. Method The separated and elucidated compounds from chloroform fractions of P. articulata Lindl were used as standards and analyzed by gradient elution HPLC with a variable wavelength detector at 274 nm. Results The calibration curves exhibited good linearity (R-2 = 0.9999), ranging from 20 to 960 ng/mL, the average recoveries were between 91.5 and 102.9%, and the RSD values of precision, stability, and repeatability were Pholidota, Dendrobium, and Bulbophyllum. Furthermore, this is the first report on the validation of a method for the quantitative analysis of flavidin and cirrhopetalidin. Conclusions The present study provides an alternative method for the rapid separation of phenanthrenes and bibenzyls from natural products and lays a foundation for the study of biological activity.