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Development of a Method for Separation Batatasin III and Cirrhopetalidin in the Stems of Bulbophyllum taeniophyllum by Using HPLC and Evaluation of Their Radical Scavenging Activity

文献类型: 外文期刊

作者: Zhu, Xiuying 1 ; Yan, Hegui 1 ; Yang, Minghui 1 ; Yang, Xiaoqiong 2 ; He, Lu 2 ;

作者机构: 1.Dali Univ, Sch Pharmaceut Sci & Chem, Dali 671000, Peoples R China

2.Yunnan Acad Agr Sci, Inst Trop Ecoagr Sci, Yuanmou 651300, Peoples R China

关键词: Bibenzyls; HPLC; Radical scavenging activity

期刊名称:CHEMISTRY LETTERS ( 影响因子:1.389; 五年影响因子:1.363 )

ISSN: 0366-7022

年卷期: 2020 年 49 卷 8 期

页码:

收录情况: SCI

摘要: Bibenzyls are one of the most essential natural ingredients for genus Bulbophyllum owing to various biological activities. Two bibenzyls (batatasin III and cirrhopetalidin) were first separated and elucidated from the stems of Bulbophyllum taeniophyllum, as well as used as standards for HPLC determination. Furthermore, the developed method was validated by performing with linearity, LODs, LOQs, precision, stability, repeatability and recovery. Then a simple and reliable HPLC-VWD method was adopted for simultaneous separation and quantitation of both analytes. In addition, the DPPH assay demonstrated that only cirrhopetalidin showed potent DPPH free radicalscavenging activity.

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