Deep eutectic solvent extraction of polysaccharides from Gastrodiae elata with ultrasonic and enzymes assistance: Process optimization, structure, and antioxidant activity
文献类型： 外文期刊
作者： Zhou, De 1 ; Hu, Yunfei 1 ; Zhou, Tianli 1 ; Peng, Shoutao 1 ; Li, Ren 1 ; He, Jinsong 1 ; Zhao, Ming 1 ; Shao, Jinliang 3 ; Tang, Qingyan 1 ;
作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Natl Local Joint Engn Res Ctr Germplasm Innovat &, Key Lab Med Plant Biol Yunnan Prov, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Characterist Plant Extract Lab Co Ltd, Kunming 650106, Yunnan, Peoples R China
3.Yunnan Acad Agr Sci, Inst Qual Stand & Testing Technol, Kunming 650200, Yunnan, Peoples R China
关键词： Deep eutectic solvent; Gastrodia elata polysaccharides; Structural characterization; Density functional theory; Antioxidant activity; HepG2 cells
期刊名称：ULTRASONICS SONOCHEMISTRY （ 影响因子：9.7； 五年影响因子：9.1 ）
ISSN： 1350-4177
年卷期： 2025 年 119 卷
页码：
收录情况： SCI
摘要： Gastrodia elata polysaccharides (GEPs) have garnered attention due to their various bioactivities; however, their current extraction yield is low, limiting widespread application. This study employed ultrasonic enzyme-assisted deep eutectic solvent (DES) extraction to enhance efficiency and compared the physicochemical properties, structure, and antioxidant activity of the polysaccharides with those obtained by traditional hot water extraction. Additionally, density functional theory (DFT) was used to explore the DES14 extraction mechanism. Results indicated that the extraction yield from the ultrasonic enzyme-assisted DES14 method (25.22 %) was 3.11 times higher than that of the hot water method (8.11 %), and the molecular weight was 0.21 times. Furthermore, the polysaccharides extracted by this method exhibited superior antioxidant activity, effectively reducing malondialdehyde and reactive oxygen species accumulation, while enhancing antioxidant enzyme activity to combat oxidative stress. Both polysaccharides were alpha-glucans linked by 4-O-D-Glcp without triple helical structures. DFT analysis demonstrated that the binding energy of DES14 with GEPs was significantly higher than with water, enhancing GEPs solubility through hydrogen bond network formation, thereby notably improving extraction yield. This study provides an efficient method for extracting natural plant polysaccharides and a theoretical basis for GEPs application in the food industry.
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