您好，欢迎访问云南省农业科学院 机构知识库！

Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway

文献类型： 外文期刊

作者： Zhao, Lei 1 ; Miao, Yue 1 ; Shan, Bo 1 ; Zhao, Chunyan 1 ; Peng, Chunxiu 3 ; Gong, Jiashun 1 ;

作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China

2.Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China

3.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China

4.Yunnan Acad Agr Sci, Agro Prod Proc Res Inst, Kunming 650223, Yunnan, Peoples R China

关键词： theabrownin; RAW264.7 macrophages; innate immune enhancement; anti-inflammatory effect; TLR2/4; NF-kappa B/MAPK/PI3K-AKT signaling pathways

期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）

ISSN：

年卷期： 2023 年 12 卷 7 期

页码：

收录情况： SCI

摘要： Theabrownin (TB) is a tea pigment extracted from Pu-erh Tea. The effects of TB on innate immunity and inflammation are not well understood. Herein, the effects of TB on innate immunity are investigated using RAW264.7 macrophages. We found that TB promoted the proliferation of RAW264.7 macrophages, altered their morphology, enhanced their pinocytic and phagocytic ability, and significantly increased their secretion of nitric oxide (NO) and cytokines, all of which enhanced the immune response. Additionally, TB inhibited the release of inflammatory signals in RAW264.7 macrophages primed with lipopolysaccharide (LPS), implying that TB modulates the excessive inflammation induced by bacterial infection. AWestern blot showed that TB could activate the toll-like receptor (TLR)2/4-mediated myeloid differentiation factor 88 (MyD88)-dependent mitogen activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) signaling pathway and the TLR2-mediated phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, enhancing the immune functions of RAW264.7 macrophages. TB also inhibited the phosphorylation of core proteins in the MAPK/NF-kappa B-PI3K-AKT signaling pathway induced by LPS. In addition, we analyzed the transcriptomes of RAW264.7 macrophages, and a Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis revealed that TB modulated thetoll-like receptor signal pathway. A gene ontology (GO) enrichment analysis indicated that TB treatment strongly modulated the immune response and inflammation. As a result, TB-enhanced innate immunity and modulated inflammation via the TLR2/4 signaling pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台