Theabrownin from Pu-erh Tea Improves DSS-Induced Colitis via Restoring Gut Homeostasis and Inhibiting TLR2&4 Signaling Pathway
文献类型: 外文期刊
作者: Zhao, Lei 1 ; Zhao, Chunyan 1 ; Miao, Yue 1 ; Lei, Shuwen 1 ; Li, Yujing 1 ; Gong, Jiashun 1 ; Peng, Chunxiu 5 ;
作者机构: 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Peoples R China
2.Yunnan Agr Univ, Coll Sci, Kunming 650201, Peoples R China
3.Yunnan Acad Agr Sci, Med Plants Res Inst, Kunming 650223, Peoples R China
4.Yunnan Acad Agr Sci, Agroprod Proc Res Inst, Kunming 650223, Peoples R China
5.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Peoples R China
关键词: Ulcerative colitis; Intestinal barrier; Differentiation of CD4+T cells; Intestinal microbiota
期刊名称:PHYTOMEDICINE ( 影响因子:6.7; 五年影响因子:6.2 )
ISSN: 0944-7113
年卷期: 2024 年 132 卷
页码:
收录情况: SCI
摘要: Background: Theabrownin (TB) is a dark brown pigment from Pu-erh tea or other dark teas. It is formed by further oxidization of theaflavins and thearubigins, in combination with proteins, polysaccharides, and caffeine etc. TB is a characteristic ingredient and bioactive substance of Pu-erh tea. However, the effects of TB on ulcerative colitis (UC) remains unclear. Purpose: This study aims to elucidate the mechanism of TB on UC in terms of recovery of intestinal homeostasis and regulation of toll-like receptor (TLR) 2&4 signaling pathway. Methods: The colitis models were established by administering 5% dextran sulfate sodium (DSS) to C57BL/6 mice for 5 days to evaluate the therapeutic and preventive effects of TB on UC. Mesalazine was used as a positive control. H&E staining, complete blood count, enzyme-linked immunosorbent assay, immunohistochemistry, flow cytometry, and 16S rRNA sequencing were employed to assess histological changes, blood cells analysis, content of cytokines, expression and distribution of mucin (MUC)2 and TLR2&4, differentiation of CD4+ T cells in lamina propria, and changes in intestinal microbiota, respectively. Western blot was utilized to study the relative expression of tight junction proteins and the key proteins in TLR2&4-mediated MyD88-dependent MAPK, NF-kappa B, and AKT signaling pathways. Results: TB outstanding alleviated colitis, inhibited the release of pro-inflammatory cytokines, reduced white blood cells while increasing red blood cells, hemoglobin, and platelets. TB increased the expression of occludin, claudin-1 and MUC2, effectively restored intestinal barrier function. TB also suppressed differentiation of Th1 and Th17 cells in the colon's lamina propria, increased the fraction of Treg cells, and promoted the balance of Treg/Th17 to tilt towards Tregs. Moreover, TB increased the Firmicutes to Bacteroides (F/B) ratio, as well as the abundance of Akkermansia, Muribaculaceae, and Eubacterium_coprostanoligenes_group at the genus level. In addition, TB inhibited the activation of TLR2&4-mediated MAPK, NF-kappa B, and AKT signaling pathways in intestinal epithelial cells of DSS-induced mice.
- 相关文献
作者其他论文 更多>>
-
Theabrownin ameliorated lipid accumulation via modulating circadian rhythms in oleic acid-induced HepG2 cells
作者:Zhao, Chunyan;Zhao, Lei;Li, Zelin;Lei, Shuwen;Shan, Bo;Wang, Qiuping;Tan, Chao;Gong, Jiashun;Li, Zelin;Gong, Jiashun;Peng, Chunxiu;Miao, Yue
关键词:Pu-erh tea; Theabrownin; Circadian rhythms; Lipid accumulation; Proteomics; siRNA; HepG2 cells
-
Caffeine regulates both osteoclast and osteoblast differentiation via the AKT, NF-κB, and MAPK pathways
作者:Miao, Yue;Lei, Shuwen;Zhao, Chunyan;Wang, Qiuping;Tan, Chao;Gong, Jiashun;Miao, Yue;Zhao, Lei;Peng, Chunxiu;Gong, Jiashun
关键词:caffeine; osteoclasts; osteoblasts; bone metabolism; ovariectomy
-
Effect of different agricultural conditions, practices, and processing on levels of total arsenic and species in cereals and vegetables: A review
作者:Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Tong, Litao;Wang, Fengzhong;Fan, Bei;Gong, Jiashun;Gong, Jiashun
关键词:Arsenic; Species; Arsenic concentration; Processing methods; Cooking methods; Agricultural products
-
Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway
作者:Zhao, Lei;Miao, Yue;Shan, Bo;Zhao, Chunyan;Gong, Jiashun;Zhao, Lei;Peng, Chunxiu;Gong, Jiashun
关键词:theabrownin; RAW264.7 macrophages; innate immune enhancement; anti-inflammatory effect; TLR2/4; NF-kappa B/MAPK/PI3K-AKT signaling pathways
-
Arsenic source analysis of rice from different growing environments and health risk assessment in Hunan Province, China
作者:Liu, Yanfang;Gong, Jiashun;Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Wang, Xinrui;Tong, Litao;Wang, Fengzhong;Fan, Bei;Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Wang, Xinrui;Tong, Litao;Wang, Fengzhong;Fan, Bei;Gong, Jiashun
关键词:Rice; Inorganic arsenic; Health risk assessment; ICP-MS; HPLC-HG-AFS; Irrigation water; Soil; Hunan Province
-
Effects of Different Probiotics on the Volatile Components of Fermented Coffee Were Analyzed Based on Headspace-Gas Chromatography-Ion Mobility Spectrometry
作者:Zhao, Linfen;Wang, Yanhua;Wang, Dongyu;He, Zejuan;Tan, Chao;Gong, Jiashun
关键词:coffee; probiotics; fermentation; aroma; headspace-gas chromatography-ion mobility spectrometry
-
Coupling RESI with Multi-Scenario LULC Simulation and Spatiotemporal Variability Analysis: An Ecological Spatial Constraint Approach
作者:Jiang, Qin;Shi, Zhengtao;Liang, Qiaoling;He, Guangxiong;Zhao, Lei;He, Li;Jiang, Qin;Shi, Zhengtao;Liang, Qiaoling;Zhao, Lei;He, Li;He, Guangxiong;He, Guangxiong
关键词:LULC; habitat quality spatial; RESI; multi-scenario; PLUS model; Yuanmou dry-hot valley