Identification, function validation and haplotype analysis of salt-tolerant genes of lectin receptor kinase gene family in sorghum (Sorghum bicolor L.)
文献类型： 外文期刊
作者： Mao, Li 1 ; He, Huazhuan 1 ; Gao, Haiyan 1 ; Huang, Wangqi 4 ; Cai, Qizhe 2 ; Yan, Guiyun 1 ; Cheng, Qingjun 1 ; Liang, Yinpei 1 ; Chen, Xiuhua 5 ;
作者机构： 1.Shanxi Agr Univ, Shanxi Key Lab Minor Crops Germplasm Innovat & Mol, Taigu, Peoples R China
2.Shanxi Agr Univ, Coll Life Sci, Taigu, Peoples R China
3.Shanxi Agr Univ, Coll Agr, Taigu, Peoples R China
4.Yunnan Acad Agr Sci, Inst Flower, Kunming, Peoples R China
5.Yunnan Acad Agr Sci, Int Agr Res Inst, Kunming, Peoples R China
关键词： L-type lectin receptor kinases; Sorghum bicolor; salt stress; function validation; haplotype
期刊名称：FRONTIERS IN GENETICS （ 影响因子：2.8； 五年影响因子：3.3 ）
ISSN：
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Introduction Salt stress is one of the significant challenges in sorghum production, greatly impacting the yield of S. bicolor. L-type lectin receptor kinases (LLRKs) are essential for plant stress tolerance, yet a comprehensive genome-level analysis in this species has not been conducted.Materials and methods Members of the SbLLRLK gene family were identified using bioinformatics methods. Gene structures, motifs, and phylogenetic relationships were analyzed. Subsequently, expression profiles under various stress conditions were examined using transcriptome data. Furthermore, functional validation was performed through homologous gene alignment and transgenic analysis, focusing on the gene SORBI_3004G304700.Results This study identified 49 SbLLRLK genes in Sorghum bicolor, categorized into four groups based on their lectin domain characteristics. Expression analyses revealed diverse patterns under salt, drought, and heat stresses. SORBI_3004G304700 was identified as a negative regulator of salt stress tolerance, with three unique haplotypes identified through haplotype analysis, suggesting a role in salt stress adaptation. Haplotype analysis of SORBI_3004G304700 revealed three unique haplotypes, with haplotype1 being the most prevalent, possibly due to selective advantages for salt stress tolerance.Discussion These findings offer valuable insights into the biological research on the role of the LLRLK gene family in Sorghum bicolor's response to salt stress. The functional characterization of SORBI_3004G304700 and the identification of haplotypes associated with salt tolerance provide valuable insights for molecular breeding and genetic enhancement of sorghum.
- 相关文献
作者其他论文 更多>>
-
Identification of the Plant Defensin (MsPDF) Gene Family in Medicago sativa and Analysis of Expression Patterns Under Abiotic Stress
作者：Guo, Meiyan;Li, Shuaixian;Shu, Yongjun;Chen, Xiuhua;Tian, Jiang;Huang, Wangqi
关键词：plant defensin; alfalfa; cold stress; expression; protein structure prediction
-
Herbicide Screening and Application Method Development for Sustainable Weed Management in Tagetes erecta L. Fields
作者：Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Zhang, Yiping;Huang, Wangqi;Xu, Feng;Jiang, Yalian;Wang, Jihua;Feng, Dongyan;Jia, Chengcheng;Tang, Dongsheng;Huang, Junhong;Li, Ye
关键词：weed management; pre-emergence herbicides; post-emergence herbicides; crop safety
-
Comparative Analysis of Chloroplast Pan-Genomes and Transcriptomics Reveals Cold Adaptation in Medicago sativa
作者：Zhang, Tianxiang;Li, Manman;Guo, Changhong;Shu, Yongjun;Chen, Xiuhua;Yan, Wei;Liu, Qian;Li, Yanan;Huang, Wangqi
关键词：alfalfa; chloroplast genome; population structure; genetic variation; cold stress
-
Comprehensive Identification of the β-Amylase (BAM) Gene Family in Response to Cold Stress in White Clover
作者：Li, Manman;Wu, Kaiyue;Bai, Yan;Guo, Donglin;Guo, Changhong;Shu, Yongjun;Chen, Xiuhua;Huang, Wangqi
关键词：white clover; BAM; cold stress; genetic regulation network; qRT-PCR
-
EMS-Mediated Mutagenesis in Marigold Seeds and Its Effects on Seedling Growth and Physiology
作者：Meng, Chao;Zhang, Yiping;Shi, Ruixue;Jiang, Yalian;Huang, Wangqi;Ullah, Ikram;Wu, Wenjin;Luo, Shaodan;Luo, Cuixia;Nanda, Satyabrata;Seleiman, Mahmoud F.
关键词：Marigolds; seed germination; EMS mutagenesis; genetic enhancement
-
Genome-Wide Identification and Expression Analysis of the Alfalfa (Medicago sativa L.) U-Box Gene Family in Response to Abiotic Stresses
作者：Li, Shuaixian;Guo, Meiyan;Zhu, Xiaoyue;Guo, Changhong;Shu, Yongjun;Chen, Xiuhua;Huang, Wangqi
关键词：alfalfa; PUB gene family; abiotic stress; expression pattern; qRT-PCR
-
Genome-wide analysis of the CDPK gene family and their important roles response to cold stress in white clover
作者：Li, Manman;Guo, Changhong;Shu, Yongjun;Chen, Xiuhua;Huang, Wangqi;Li, Yanan;Liu, Qian;Yan, Wei;Yan, Wei
关键词：White clover; CDPK; Genetic regulation network; Cold stress; RNA-seq
