Identification of the Plant Defensin (MsPDF) Gene Family in Medicago sativa and Analysis of Expression Patterns Under Abiotic Stress
文献类型： 外文期刊
作者： Guo, Meiyan 1 ; Chen, Xiuhua 2 ; Li, Shuaixian 1 ; Tian, Jiang 2 ; Huang, Wangqi 3 ; Shu, Yongjun 1 ;
作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol Cytogenet & Genet Breeding Heilongjian, Harbin 150025, Peoples R China
2.Yunnan Acad Agr Sci, Int Agr Res Inst, Kunming 650200, Peoples R China
3.Yunnan Acad Agr Sci, Flower Res Inst, Natl Engn Res Ctr Ornamental Hort, Yunnan Flower Breeding Key Lab, Kunming 650200, Peoples R China
关键词： plant defensin; alfalfa; cold stress; expression; protein structure prediction
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 9 期
页码：
收录情况： SCI
摘要： Medicago sativa L. (alfalfa) is a major forage crop due to its high yield and stress resilience. However, its growth and productivity are often compromised by abiotic stresses, including cold, drought, and salinity. The plant defensin (PDF) gene family plays a crucial role in resistance to abiotic stress. In this study, a total of 11 MsPDF gene family members were identified in the alfalfa genome and classified into three groups. Phylogenetic and conserved motif analyses revealed that the MsPDF genes are highly conserved. Promoter analysis, gene regulatory network analysis (GRN), and gene ontology (GO)-enrichment analyses were used to infer the potential functions of MsPDF genes. The results showed that the gene actively responds to abiotic stress, participates in phytohormonal responses, and regulates plant growth and development through gene interactions. Transcriptome and qRT-PCR analyses showed that most of the MsPDF genes were significantly up-regulated under cold, drought, and salinity stresses. Among them, the MsPDF03 exhibited superior performance under cold stress. The MsPDF04, MsPDF08, and MsPDF09 genes were able to respond positively to drought and salt stresses. Finally, the monomeric, dimeric, and tetrameric structures of the proteins encoded by the MsPDF genes were predicted using AlphaFold 2 software. This study lays the foundation for the identification and evolutionary relationship analysis of the MsPDF gene family, and provides a new reference for subsequent research on abiotic stress resistance.
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