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An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica

文献类型： 外文期刊

作者： Zhao Shuang 1 ; Zhao, Yong Chang 4 ; Li, Shu Hong 4 ; Zhang, Guo Qing 1 ; Wang, He Xiang 1 ; Ng, Tzi Bun 5 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

4.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming 650223, Peoples R China

5.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China

关键词： Antiproliferative;Purification;Ribonuclease;Russula delica

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

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收录情况： SCI

摘要： An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and 60°C, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C>poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an IC_(50) value of 8.6 μM and 7.2 μM, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.

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