您好，欢迎访问云南省农业科学院 机构知识库！

Determining the Active Ingredients of Dendrobium officinale and Tracing Its Region of Origin

文献类型： 外文期刊

作者： Zhang, Jiao 1 ; Wang, An 2 ; Wei, Xiao 3 ; Liu, Zhenhuan 1 ; Hu, Zhengxu 4 ; Wang, Jing 4 ; Chen, Xinglian 1 ; Du, Lijuan 1 ; Liu, Hongcheng 1 ; Lin, Tao 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Qual Stand & Testing Technol Res Inst, Kunming 650205, Peoples R China

2.Yunnan Univ Tradit Chinese Med, Affiliated Hosp 1, Kunming 650021, Peoples R China

3.Yunnan Normal Univ, Coll Vocat & Tech Educ, Kunming 650092, Peoples R China

4.Longling Cty Agr Technol Extens Ctr, Longling 678300, Peoples R China

关键词： Dendrobium officinale; Traceability; Geographical origin discrimination; Active ingredients

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.0； 五年影响因子：3.1 ）

ISSN： 1936-9751

年卷期： 2025 年 18 卷 8 期

页码：

收录情况： SCI

摘要： A method for the determination of 22 active ingredients, including flavonoids, phenols, and glycosides, in Dendrobium officinale was established using UHPLC-MS/MS. The 22 active compounds presented good linear correlation coefficients in the concentration range of 0.005 and 10 mu gmL-1, limits of detection and quantification ranging between 0.008 and 2.25 mgkg-1 and 0.025 and 7.5 mgkg-1, respectively, and interday and intraday RSDs ranging between 0.14 and 12.57% and 0.08 and 9.92%, respectively, and RE values ranging between 0.01 and 9.57% and 0.02 and 9.33%, respectively. Moreover, the detection sensitivity and stability of the method were good. D. officinale samples from different regions in Yunnan (Xishuangbanna, Baoshan, Dehong, and Wenshan) were effectively distinguished using OPLS-DA on the basis of the contents of 22 compounds, and ten differentially abundant metabolites, such as syringin, scutellarein, gallic acid, and gentisic acid, were identified, which can be used to identify the origin of D. officinale to some extent. The samples from Dehong presented relatively high levels of syringin, gentisic acid, protocatechuic acid, and gallic acid; the samples from Baoshan contained relatively high levels of apigenin, naringin, and 4-hydroxybenzaldehyde; the samples from Wenshan exhibited relatively high levels of epicatechin gallate, scopoletin, and scutellarein; and the samples from Xishuangbanna presented relatively low levels of protocatechuic acid and gallic acid. The accumulation of active ingredients was affected by the D. officinale growth environment. There were no significant differences in the contents of epicatechin gallate, scopoletin, or scutellarein in the samples from the Dehong, Baoshan, and Xishuangbanna regions or in the content of apigenin in the samples from the Dehong and Xishuangbanna regions; however, there were large significant differences in the contents of all other differentially abundant metabolites between samples from the Wenshan region and those from the other three regions. This method is simple and effective and can provide technical support to trace the origin of Dendrobium species and other agricultural products.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台