The Rapid Determination of Artemisinin by Post-Column Derivatization High-Performance Liquid Chromatography Using Matrix Solid-Phase Dispersion Method
文献类型： 外文期刊
作者： Liu, Hongcheng 1 ; Li, Qiwan 1 ; Li, Shaoping 2 ; Zou, Yanhong 1 ; Gu, Anyun 2 ;
作者机构： 1.Minist Agr, Yunnan Acad Agr Sci, Qual Stand & Testing Technol Inst, Kunming Supervis & Testing Ctr Farm Prod Qual, Kunming 650223, Peoples R China
2.Med Plants Res Inst, Kunming 650223, Peoples R China
期刊名称：JOURNAL OF CHROMATOGRAPHIC SCIENCE （ 影响因子：1.618； 五年影响因子：1.682 ）
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收录情况： SCI
摘要： Artemisinin (an antimalaric compound) is isolated as the active compound of the medicinal plant Artemisia annua L. A simple, rapid, and high-efficient method of extraction is developed, in which it is extracted by matrix solid-phase dispersion (MSPD) and directly analyzed by post-column derivatization high-performance liquid chromatography (HPLC). The quantitation results from the MSPD method are compared with two conventional liquid solvent extraction processes, Soxhlet and ultrasonic wave by F-test, and the result indicates no significant difference. The production rate of Artemisinin during wild plant growth (tested over two years, acquired from Yunnan, provincial Yuan Yan country) is determined. The recovery range of determination is 88.1-91.2%. The relative standard deviation (RSD) is 4.55-6.43%. The limits of detection are 0.1 μg/mL, and the limits of quantitation are 0.5 μg/mL.
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