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A Combination of Ultrasonic-assisted Extraction with RRLC-QQQ Method for the Determination of Artemisinin in the Chinese Herb Artemisia annua L.

文献类型： 外文期刊

作者： Wang, Xiao 1 ; Zhao, Xian-en 2 ; Yang, Bin 1 ; Dong, Hongjing 2 ; Liu, Dahui 1 ; Huang, Luqi 1 ;

作者机构： 1.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

2.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China

3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650231, Peoples R China

关键词： artemisinin: 63968-64-9;antiinfective-drug;antiparasitic-drug;methanol-water

期刊名称：PHYTOCHEMICAL ANALYSIS （ 影响因子：3.373； 五年影响因子：2.959 ）

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收录情况： SCI

摘要： Introduction - Artemisinin, the primary active ingredient of the Chinese herb Artemisia annua L., is known to have considerable anti-malaria properties. However, rapid, sensitive and selective method for the determination of artemisinin in it is not currently available.Objective - To develop and validate an efficient method for extraction and analysis of artemisinin from the plant samples of Artemisia annua L by rapid resolution liquid chromatography triple quadrupole mass spectrometry (RRLC-QQQ).Methodology - Following ultrasound-assisted extraction (USE), RRLC-QQQ was utilised to separate and determine artemisinin from the plant sample of Artemisia annua L. The LC separation, QQQ-MS detection and multiple reaction monitoring (MRM) mode were optimised, and the method validation concluding selectivity, calibration, accuracy and precision, and recovery were also evaluated.Results - LC separation was performed with an isocratic elution of 20% of methanol-water (10 mmol/L ammonium acetate, pH 4.0) on a C-18 column. The triple quadrupole MS detection was carried out under MRM mode of precursor ion [M + H](+) -> fragment ions m/z 265.1 and m/z 247.2. The limits of detection and quantitation of artemisinin were 0.20 and 0.75 ng/ml, respectively. The intra- and inter-day precisions did not exceed 3.71%, and the deviation of the intra- and inter-day mean values did not exceed +/- 7.50. The average recoveries for artemisinin ranged from 92.45 to 103.8% with an RSD from 2.47 to 2.79%.Conclusion - The developed RRLC-QQQ assay is an efficient method for separation and determination of artemisinin from the plant samples of Artemisia annua L. Copyright (C) 2011 John Wiley & Sons, Ltd.

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