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Purification and characterization of a novel laccase from the mushroom pleurotus nebrodensis

文献类型： 外文期刊

作者： Tian, Guo-Ting 5 ; Zhang, Guo-Qing 4 ; Wang, He-Xiang 1 ; Ng, Tzi Bun 3 ;

作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

2.China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

3.Chinese Univ Hong Kong, Fac Med, Dept Biochem, Shatin, Hong Kong, Peoples R China

4.Beijing Agr Univ, Key Lab Urban Agr N, Minist Agr, Beijing 100094, Peoples R China

5.Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resource, Kunming, Peoples R China

关键词： Characterization;Laccase;Pleurotus nebrodensis;Purification

期刊名称：ACTA BIOCHIMICA POLONICA （ 影响因子：2.149； 五年影响因子：2.175 ）

ISSN：

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收录情况： SCI

摘要： A novel laccase with a molecular mass of 64 kDa and the N-terminal sequence AIGPDDTINF was isolated from fresh fruiting bodies of the mushroom Pleurotus nebrodensis. The purification protocol comprised ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and QSepharose, but not on CM-cellulose. It demonstrated an optimal temperature of 70°C. The enzyme activity increased steadily over the temperature range 20°C-70°C. There was only a slight reduction in activity at 80°C. However, all activity disappeared following exposure to 100°C for 10 minutes. The enzyme activity changed only slightly over the pH range 3-5, with the optimum at pH 5, but underwent a precipitous decline when the pH was elevated to 6, and was undetectable at pH 8 and pH 9.

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