您好，欢迎访问云南省农业科学院 机构知识库！

Synergistic effects of ultraviolet-B and methyl jasmonate on tanshinone biosynthesis in Salvia miltiorrhiza hairy roots

文献类型： 外文期刊

作者： Wang, Cong Hui 1 ; Zheng, Li Ping 2 ; Tian, Hao 1 ; Wang, Jian Wen 1 ;

作者机构： 1.Soochow Univ, Coll Pharmaceut Sci, Suzhou 215123, Peoples R China

2.Soochow Univ, Dept Hort, Suzhou 215123, Peoples R China

3.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650205, Peoples R China

关键词： Salvia miltiorrhiza;Hairy roots;Tanshinones;UV-B;MeJA;Elicitation

期刊名称：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY （ 影响因子：6.252； 五年影响因子：5.375 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Tanshinones are major bioactive diterpenoids of Salvia miltiorrhiza roots used for the treatment of cardiocerebral diseases. To develop effective elicitation and bioprocess strategies for the enhanced production of tanshinones, ultraviolet-B (UV-B) irradiation and methyl jasmonate (MeJA) elicitation were applied alone or in combination respectively in S. miltiorrhiza hairy root cultures. Our results showed 40-min UV-B irradiation at 40 mu W/cm(2) stimulated tanshinone production without any suppression of root growth, suggesting a new effective elicitor to S. miltiorrhiza hairy root cultures for tanshinone production. Moreover, the combined treatment of UV-B irradiation and MeJA exhibited synergistic effects on the expression levels of 3-hydroxy-3-methylglutrayl-CoA reductase (SmHMGR) and geranylgeranyl diphosphate synthase (SmGGPPS) genes in the tanshinone biosynthetic pathway. When hairy roots of 18-day-old cultures were exposed to the combined elicitation for 9 days, the maximum production of tanshinone reached to 28.21 mg/L, a 4.9-fold increase over the control. The combined elicitation of UV-B and MeJA was firstly used to stimulate the production of biologically important secondary metabolites in hairy root cultures. (C) 2016 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Cloning and characterization of an elicitor-responsive gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase involved in 20-hydroxyecdysone production in cell cultures of Cyanotis arachnoidea. Wang, Qiu Jun,Zhao, Yi Lu,Wang, Jian Wen,Zheng, Li Ping,Zhao, Pei Fei.

[2]Stimulation of Artemisinin Production in Artemisia annua Hairy Roots by Ag-SiO2 Core-shell Nanoparticles. Zhang, Ben,Wang, Jian Wen,Zheng, Li Ping,Li, Wan Yi. 2013

[3]Effects of LaCl3 on photosynthesis and the accumulation of tanshinones and salvianolic acids in Salvia miltiorrhiza seedlings. Zhou Jie,Guo Lanping,Yang Guang,Zhao Manxi,Huang Luqi,Zhang Ji,Zhou Shufeng,Zhou Shufeng. 2011

[4]Inoculation with Glomus mosseae Improves the Growth and Salvianolic Acid B Accumulation of Continuously Cropped Salvia miltiorrhiza. Chen, Meilan,Yang, Guang,Guo, Lanping,Huang, Luqi,Liu, Dahui,Li, Minhui,Qiu, Hongyan,Chao, Zhi. 2017

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台