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Proteomic analysis reveals growth inhibition of soybean roots by manganese toxicity is associated with alteration of cell wall structure and lignification

文献类型： 外文期刊

作者： Chen, Zhijian 1 ; Yan, Wei 1 ; Sun, Lili 1 ; Tian, Jiang 1 ; Liao, Hong 1 ;

作者机构： 1.South China Agr Univ, Root Biol Ctr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Crop Genet Resources, Haikou 571101, Peoples R China

3.Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan 678000, Peoples R China

4.Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Root Biol Ctr, Fuzhou 350000, Peoples R China

5.Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Root Biol Ctr, Fuzho

关键词： Root;Mn toxicity;Proteomic analysis;Soybean

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）

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收录情况： SCI

摘要： Plant roots, the hidden half of plants, play a vital role in manganese (Mn) toxicity tolerance. However, molecular mechanisms underlying root adaptation to Mn toxicity remain largely unknown. In this study, soybean (Glycine max) was used to investigate alterations of root morphology and protein profiles in response to Mn toxicity. Results showed that soybean root growth was significantly inhibited by Mn toxicity. Subsequent proteomic analysis revealed that 31 proteins were successfully identified via MALDI TOF/TOF MS analysis including 21 unique up regulated and 6 unique down-regulated proteins, which are mainly related to cell wall metabolism, protein metabolism and signal transduction. qRT-PCR analysis revealed that corresponding gene transcription patterns were correlated with accumulation of 14 of 21 up-regulated proteins, but only 1 of 6 down-regulated proteins, suggesting that most excess Mn up-regulated proteins are controlled at the transcriptional levels, while down-regulated proteins are controlled at the post-transcriptional levels. Furthermore, changes in abundances of GTP-binding nuclear protein Ran-3, expansin-like B1-like protein, dirigent protein and peroxidase 5-like protein strongly suggested that alteration of root cell wall structure and lignification might be associated with inhibited root growth. Taken together, this study was helpful to further understandings of adaptive strategies of legume roots to Mn toxicity.

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