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Ultrasensitive chemiluminescence of tetracyclines in the presence of MCLA

文献类型： 外文期刊

作者： Zeng, Wangsheng 1 ; Zhu, Chenyao 1 ; Liu, Hongcheng 2 ; Liu, Jing 1 ; Cai, Hongping 1 ; Cheng, Xianglei 1 ; Wei, Lijun 1 ;

作者机构： 1.Nanchang Univ, Sch Publ Hlth, Nanchang 330006, Jiangxi, Peoples R China

2.Yunnan Acad Agr Sci, Inst Qual Standard & Testing Technol, Kunming 650223, Peoples R China

关键词： Chemiluminescence;Tetracyclines;MCLA

期刊名称：JOURNAL OF LUMINESCENCE （ 影响因子：3.599； 五年影响因子：3.006 ）

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收录情况： SCI

摘要： Abstract In this article, five tetracyclines (TCs) showed ultrasensitive chemiluminescence (CL) based on the novel CL system of methoxylated Cypridina luciferin analogues (MCLA) and Ce(IV). It was found that the CL intensity of MCLA-Ce(IV) system was enhanced 30 times by adding 10 pmol chlortetracycline. Mechanisms of MCLA-Ce(IV)-TCs were investigated by CL spectra, radical scavengers and UV spectra. The results indicated that singlet oxygen, resulting from the reaction between Ce(IV) and TCs, was involved in the enhancement of CL phenomenon. This CL system coupling with flow injection analysis was developed for the determination of five common TCs. Linear ranged from 100fmol to 20pmol (r>0.999) with limit of detection of 20 to 50fmol (S/N=3). The sensitivity of the present method is comparable to that of UPLC-MS/MS detection for TCs. ]]>

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