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Expression pattern and tissue localization of the class B scavenger receptor BmSCRBQ4 in Bombyx mori

文献类型： 外文期刊

作者： Dong, Zhan-Peng 1 ; Chai, Chun-Li 3 ; Dai, Fang-Yin 2 ; Pan, Min-Hui 2 ; Huang, Ping 1 ; Wang, Wei 4 ; Liao, Peng-Fei; 1 ;

作者机构： 1.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi, Yunnan, Peoples R China

2.Southwest Univ, Inst Sericulture & Syst Biol, Chongqing 400716, Peoples R China

3.Southwest Univ, Coll Biotechnol, Chongqing 400716, Peoples R China

4.Southwest Univ, Coll Anim Sci & Technol, Chongqing 400716, Peoples R China

5.Southwest Univ, Coll Anim Sci & Technol, Chongqing 400716, Peoples R

关键词： BmSCRBQ4;Bombyx mori;Cameo2;class B scavenger receptor;gene expression;immunohistochemistry

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2015 年 22 卷 6 期

页码：

收录情况： SCI

摘要： Class B scavenger receptors (SR-Bs) are cell surface glycoproteins involved in various physiological processes in vivo, including the transport and metabolism of lipids, binding and phagocytosis of xenobiotics, and signaling. But little information is available about silkworm SR-Bs; it is necessary to study these SR-Bs for revealing their function. In this study, we cloned the full-length coding sequence of BmSCRBQ4, a SR-B gene from the silkworm Bombyx mori L. We found that the BmSCRBQ4 gene consists of nine exons and eight introns, with an open reading frame of 1371 bp encoding 456 amino acids. Gene expression studies determined that BmSCRBQ4 messenger RNA (mRNA) was expressed in unfertilized eggs, during embryonic development and throughout the majority of the larval period. Expression of mRNA was detected in the mid gut, middle silk gland, posterior silk gland, head, integumentum, fat body, testes and the ovaries of the larval B. mori Dazao strain, as well as in the silkworm cell lines BmN and BmE. Protein expression studies found BmSCRBQ4 protein was expressed only in the testes, fat body and middle silk gland of larvae, as well as in the silkworm cell lines BmN and BmE. The BmSCRBQ4 protein showed variability in banding patterns in different tissues and cells when analyzed by Western blotting. Immunohistochemical staining showed that the BmSCRBQ4 protein localizes to the constitutive membranes or cellular membranes of these tissues. These results indicated that BmSCRBQ4 gene may play some physiologically relevant roles at the cell surface in each tissue.

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