文献类型： 外文期刊
作者： Li, Yan 1 ; Gao, Lian-Ming 1 ; Poudel, Ram C. 1 ; Li, De-Zhu 1 ; Forrest, Alan 5 ;
作者机构： 1.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Peoples R China
2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Peoples R China
3.Chinese Acad Sci, Germplasm Bank Wild Species SW China, Kunming Inst Bot, Kunming 650204, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Royal Bot Garden Edinburgh, Edinburgh EH3 5LR, Midlothian, Scotland
关键词： DNA barcoding;matK;gymnosperm;primer universality
期刊名称：JOURNAL OF SYSTEMATICS AND EVOLUTION （ 影响因子：4.098； 五年影响因子：4.735 ）
ISSN： 1674-4918
年卷期： 2011 年 49 卷 3 期
页码：
收录情况： SCI
摘要： DNA barcoding is a tool to provide rapid and accurate taxonomic identification using a standard DNA region. A two-marker combination of matK+rbcL was formally proposed as the core barcode for land plants by the Consortium for the Barcode of Life Plant Working Group. However, there are currently no barcoding primers for matK showing high universality in gymnosperms. We used 57 gymnosperm species representing 40 genera, 11 families and four subclasses to evaluate the universality of nine candidate matK primers and one rbcL primer in this study. Primer (1F/724R) of rbcL is proposed here as a universal primer for gymnosperms due to high universality. One of the nine candidate matK primers (Gym_F1A/Gym_R1A) is proposed as the best "universal" matK primer for gymnosperms because of high polymerase chain reaction success and routine generation of high quality bidirectional sequences. A specific matK primer for Ephedra was newly designed in this study, which performed well on the sampled species. The primers proposed here for rbcL and matK can be easily and successfully amplified for most gymnosperms.
- 相关文献
作者其他论文 更多>>
-
Preparing Colour-Tunable Tannic Acid-Based Carbon Dots by Changing the pH Value of the Reaction System
作者：Li, Yan;Liu, Can;Chen, Menglin;Zheng, Yunwu;Lin, Xu;Li, Yan;Shi, Rui;He, Xiahong;Tian, Hao
关键词：biomass CDs; solvothermal method; TA; o-phthalaldehyde; W-LED
-
Preparation of Multicolour Solid Fluorescent Carbon Dots for Light-Emitting Diodes Using Phenylethylamine as a Co-Carbonization Agent
作者：An, Yulong;Liu, Can;Li, Yan;Chen, Menglin;Zheng, Yunwu;Lin, Xu;Liu, Can;Shi, Rui;He, Xiahong;Lin, Xu;Tian, Hao
关键词：carbon dots; solid fluorescence; solvent control; multicolour; optoelectronic applications
-
Solvent-Free Preparation of Tannic Acid Carbon Dots for Selective Detection of Ni2+ in the Environment
作者：Li, Yan;Lin, Xu;Li, Yan;Shi, Rui;He, Xiahong;Lin, Xu;Liu, Can;Chen, Menglin;An, Yulong;Zheng, Yunwu;Tian, Hao
关键词：biomass carbon dots; solvent-free; tannic acid; Ni2+ detection
-
Preparation of solvent-free starch-based carbon dots for the selective detection of Ru3+ ions
作者：Chen, Menglin;Lin, Xu;Chen, Menglin;Shi, Rui;He, Xiahong;Lin, Xu;Liu, Can;Zhai, Jichao;An, Yulong;Li, Yan;Zheng, Yunwu;Tian, Hao
关键词：
-
Solvent-Free Pyrolysis Strategy for the Preparation of Biomass Carbon Dots for the Selective Detection of Fe3+ Ions
作者：Chen, Menglin;Lin, Xu;Chen, Menglin;Shi, Rui;He, Xiahong;Lin, Xu;Zhai, Jichao;An, Yulong;Li, Yan;Zheng, Yunwu;Liu, Can;Tian, Hao
关键词：solvent-free pyrolysis; lignin; cellulose; biomass carbon dots; metal ion detection
-
Application of high-efficiency green fluorescent carbon dots prepared by acid catalysis in multicolour LEDs
作者：An, Yulong;Liu, Can;Li, Yan;Chen, Menglin;Zheng, Yunwu;Shi, Rui;He, Xiahong;Lin, Xu;Liu, Can;Zheng, Yunwu;Lin, Xu;Tian, Hao
关键词：
-
Dietary fresh lemon improves the albumen quality, immune status and lipid metabolism of Jingfen laying hens during the late laying period
作者：Wan, Yi;Ma, Ruiyu;Qi, Renrong;Li, Yan;Liu, Wei;Li, Junying;Zhan, Kai;Liu, Lulu;Zheng, Jiangxia;Yue, Jianqiang
关键词：Fresh lemon; production performance; egg quality; serum parameter; laying hen
