文献类型： 外文期刊
作者： Xie, Y 1 ; Zhou, XP 2 ; Zhang, ZK 2 ; Qi, YJ 2 ;
作者机构： 1.Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China
2.Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China; Yunnan Acad Agr Sci, Yunnan Biotechnol Res Inst, Kunming 650223, Peoples R China
关键词： tobacco curly shoot virus;Begomovirus;distinct species;recombination
期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）
ISSN： 1001-6538
年卷期： 2002 年 47 卷 3 期
页码：
收录情况： SCI
摘要： Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98 % amino acid sequence identity).
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