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Molecular characterization of a novel defective DNA isolated from tobacco tissues infected with tobacco leaf curl virus

文献类型： 外文期刊

作者： Zhou, XP 1 ; Xie, Y 2 ; Zhang, ZK 2 ; Qi, YJ 2 ; Wu, JJ 2 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China

2.Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China; Yunnan Acad Agr Sci, Yunnan Biotechnol Res Inst, Kunming, Peoples R China

关键词： tobacco leaf curl virus;defective DNA;deletion;encapsidation;transmission

期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）

ISSN： 0001-723X

年卷期： 2001 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Defective DNA of tobacco leaf curl virus (TLCV) was identified in TLCV-infected tobacco plants. The defective DNA was cloned and sequenced. The sequence showed it was about half the size of the TLCV DNA-A, and was derived from TLCV DNA-A by a large deletion. The defective DNA contained the intergenic region and part of the AC I (Rep) gene of TLCV, and also novel open reading frames (ORFs). The immunotrapping tests showed the defective DNA was associated with geminate particles, suggesting it could be encapsidated in virus particles. it was transmitted, along with full-length DNA-A, to tobacco plants by grafting and whitefly (Bemisia tabaci).

  • 相关文献

[1]Identification of a novel DNA molecule associated with Tobacco leaf curl virus. Xie, Y,Zhou, XP,Li, ZH,Zhang, ZK,Li, GX. 2002

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