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Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

文献类型: 外文期刊

作者: Zhao, Kuangjie 1 ; Yin, Yueyan 2 ; Hua, Mengying 1 ; Wang, Shaoxiang 4 ; Mo, Xiaohan 5 ; Yuan, Enping 4 ; Zheng, Hongying 1 ; Lin, Lin 1 ; Chen, Hairu 3 ; Lu, Yuwen 1 ; Chen, Jianping 1 ; Peng, Jiejun 1 ; Yan, Fei 1 ;

作者机构: 1.Ningbo Univ, Inst Plant Virol, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Zhejiang, Peoples R China

2.Yunnan Acad Agr Sci, Inst Alpine Econ Plants, Lijiang 674100, Yunnan, Peoples R China

3.Yunnan Agr Univ, Coll Plant Protect, Kunming 650201, Yunnan, Peoples R China

4.Wenshan Acad Agr Sci, Wenshan 663000, Yunnan, Peoples R China

5.Yunnan Acad Tobacco Agr Sci, Kunming 650021, Yunnan, Peoples R China

关键词: Pepper vein yellows virus; Recombination; Polerovirus; Readthrough domain

期刊名称:VIROLOGY JOURNAL ( 影响因子:4.099; 五年影响因子:3.719 )

ISSN:

年卷期: 2021 年 18 卷 1 期

页码:

收录情况: SCI

摘要: Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5 ' half of its genome (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3 '-part encoding P5, where it is most similar (91.7% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event affecting nucleotide positions 4126 to 5192 nt, with PeVYV-3 as the major parent but with the region 4126-5192 nt derived from TVDV as the minor parent. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.

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