您好，欢迎访问云南省农业科学院 机构知识库！

Calotropis gigantea extract induces apoptosis through extrinsic/intrinsic pathways and reactive oxygen species generation in A549 and NCI-H1299 non-small cell lung cancer cells

文献类型： 外文期刊

作者： Lee, Jiyon 1 ; Jang, Hui-Ju 1 ; Chun, Hyunwoo 1 ; Pham, Thu-Huyen 1 ; Bak, Yesol 1 ; Shin, Jong-Woon 1 ; Jin, Hang 1 ; Ki 1 ;

作者机构： 1.Konkuk Univ, Dept Biosci & Biotechnol, Lab Cell Biol & Immunobiochem, 120 Neungdong Ro, Seoul 05029, South Korea

2.YAAS, Inst Med Plants, 2238 Beijing Rd, Kunming 650205, Yunnan, Peoples R China

3.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, 125 Kuahak Ro, Daejeon 34141, South Korea

4.Korea Res Inst Biosci & Biotechnol, Nat Med Res Ctr, 30 Yeongudanji Ro, Cheongju 28116, South Korea

5.Konkuk Univ, Dept Biosci & Biotechnol, 120 Neungdong Ro, Seoul 05029, South Korea

关键词： Calotropis gigantea; Non-small cell lung cancer cell; Anti-cancer; Apoptosis; ROS

期刊名称：BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：3.659； 五年影响因子：3.767 ）

ISSN： 1472-6882

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundCalotropis gigantea (CG) is a tall and waxy flower that is used as a traditional remedy for fever, indigestion, rheumatism, leprosy, and leukoderma. However, the precise mechanisms of its anticancer effects have not yet been examined in human non-small cell lung cancer (NSCLC) cells. In this study, we investigated whether CG extract exerted an apoptotic effect in A549 and NCI-H1299 NSCLC cells.MethodsThe ethanol extract of CG was prepared, and its apoptotic effects on A549 and NCI-H1299 NSCLC cells were assessed by using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, annexin V-fluorescein isothiocyanate/propidium iodide (PI) staining, cell cycle analysis, real-time polymerase chain reaction (RT-PCR), western blotting, JC-1 staining, and ROS detection assay.ResultsThe CG extract induced apoptosis through the stimulation of intrinsic and extrinsic signaling pathways in A549 and NCI-H1299 lung cancer cells. Cell cycle arrest was induced by the CG extract in both cell lines. Reactive oxygen species (ROS), which can induce cell death, were also generated in the CG-treated A549 and NCI-H1299 cells.ConclusionsThese data confirmed that CG caused apoptosis through the activation of extrinsic and intrinsic pathways, cell cycle arrest, and ROS generation in A549 and NCI-H1299 lung cancer cells. Thus, CG can be suggested as a potential agent for lung cancer therapy.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台