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Verification of serological relationship between two phylogenetically related peanut-infecting Tospovirus species

文献类型： 外文期刊

作者： Kang, Ya-Chi 1 ; Yeh, Shyi-Dong 2 ; Liao, Chih-Hsuan 1 ; Chou, Wan-Chen 1 ; Liu, Fang-Lin 2 ; Dong, Jia-Hong 4 ; Chen, 1 ;

作者机构： 1.Asia Univ, Dept Biotechnol, Taichung 41354, Taiwan

2.Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 40227, Taiwan

3.Natl Chung Hsing Univ, NCHU UCD Plant & Food Biotechnol Program, Ctr Biotechnol, Taichung 40227, Taiwan

4.Yunnan Acad Agr Sci, Yunnan Prov Key Lab Agr Biotechnol, Key Lab Southwestern Crop Gene Resources & Germpl, Minist Agr Biotechnol & Germplasm Resources, Kunming 650223, Peoples R China

5.China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung 40402, Taiwan

关键词： Tospovirus;Nucleocapsid protein;Antibody;Serogroup

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

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收录情况： SCI

摘要： Based on the serological relationships of nucleocapsid proteins (NPs), a tospovirus species can be classified as a member of a serogroup or a distinct serotype, which greatly helps virus identification and disease diagnosis. Recent studies reported that distinct tospovirus species sharing above 51.8 % amino acid (aa) identity in their NPs may be serologically related. Two phylogenetically related peanut-infecting tospovirus species, Peanut chlorotic fan-spot virus (PCFV) in Taiwan and Peanut yellow spot virus (PYSV) in India, were previously considered as distinct serotypes, since no serological relationship has been established. To verify the serological relationship of PCFV and PYSV, the NP of PCFV was purified from leaf tissues of the infected Chenopodium quinoa plants and used to produce polyclonal antiserum (RAs-PCFV NP) and a monoclonal antibody (MAb-PCFV NP). Polyclonal antiserum to the bacterially expressed NP of PYSV (RAs-PYSV NP) was also prepared. RAs-PCFV NP reacted with the homologous PCFV NP and the bacterial-expressed PYSV NP and tissue extracts of PYSV-infected plants. Reciprocally, RAs-PYSV NP reacted with the homologous bacterial-expressed PYSV NP and the tissue extracts of PYSV-infected or PCFV-infected plants. In addition, MAb-PCFV NP reacted only with the tissue extracts of PCFV-infected plants. Our results demonstrate that PCFV NP is serologically related to PYSV NP and they should be classified as members of a unique serogroup.

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