您好，欢迎访问云南省农业科学院 机构知识库！

Characterization and expression analysis of cDNAs encoding abscisic acid 8'-hydroxylase during mulberry fruit maturation and under stress conditions

文献类型： 外文期刊

作者： Cai, Yuxiang 1 ; Zhu, Panpan 1 ; Liu, Changying 1 ; Zhao, Aichun 1 ; Yu, Jian 1 ; Wang, Chuanhong 1 ; Li, Zhengang 2 ; Hu 1 ;

作者机构： 1.Southwest Univ, Coll Biotechnol, Chongqing 400715, Peoples R China

2.Yunnan Acad Agr Sci, Sericultural & Apicultural Res Inst, Mengzi 661100, Yunnan, Peoples R China

关键词： Mulberry;ABA catabolism;CYP707A;80-hydroxylase;Uniconazole;Drought;Salt stress

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) 8'-hydroxylase is the key enzyme in the oxidative catabolism of ABA. CYP707A genes encode (+)-ABA 8'-hydroxylases that are involved in fruit ripening and abiotic stress responses. However, there are limited reports on the functions of CYP707A genes in mulberry. This study analyzed the transcriptional expression of CYP707A genes during fruit development and their response to exogenous ABA and uniconazole (UNI) treatment. Additionally, the expression patterns of CYP707A under stress conditions were explored in mulberry. We obtained six CYP707A gene (MnCYP707A1-MnCYP707A6) sequences from Morus notabilis genome database. Multiple sequence alignment results showed high conservation within the MnCYP707A genes. The expression profiles of these genes during fruit development and under stress conditions were analyzed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). During fruit development, the expression levels of MaCYP707A1, MaCYP707A3, MaCYP707A5, and MaCYP707A6 were higher at the primary stage and then sharply declined to a lower level at the late stage. The expression of MaCYP707A1 in the fruit quickly declined at 1 d after 50 mmol/L UNI treatment. In addition, under Sodium chloride (NaCl) (0.3 % (w/v) at germination stage and 0.6 % (w/v) at seedling stage) or polyethylene glyco (PEG) 6000 (5 % (w/v) at germination stage and 20 % (w/v) at seedling stage) stress, most of the MaCYP707A genes were up-regulated, but displayed different patterns. Tissue-specific patterns of the CYP707A family appear to be universal under stress conditions. In a short, MaCYP707A genes play overlapping roles in ABA catabolism and MaCYP707A1 may be the key gene expressed during fruit maturation. Most of these genes were induced by abiotic stress, and various expression patterns were observed among the MaCYP707A in multiple mulberry tissues at different growth periods.

  • 相关文献

[1]Transcriptional Regulation of Genes Encoding Key Enzymes of Abscisic Acid Metabolism During Melon (Cucumis melo L.) Fruit Development and Ripening. Sun, Yufei,Chen, Pei,Duan, Chaorui,Wang, Yanping,Ji, Kai,Hu, Yin,Li, Qian,Dai, Shengjie,Wu, Yan,Luo, Hao,Sun, Liang,Leng, Ping,Tao, Pang. 2013

[2]Development of a specific molecular tool for the detection of epidemiologically active mulberry causing-disease strains of Ralstonia solanacearum phylotype I (historically race 5-biovar 5) in China. Xu, J.,Xu, J. S.,Zhang, H.,Chen, K. Y.,Tian, Q.,Zhang, L. Q.,Liu, L.,He, L. Y.,Feng, J.,Prior, P.,Pan, Z. C..

[3]Isolation and characterization of a novel chalcone synthase gene family from mulberry. Wang, Chuanhong,Zhi, Shuang,Liu, Changying,Xu, Fengxiang,Zhao, Aichun,Wang, Xiling,Tang, Xing,Yu, Maode,Wang, Chuanhong,Zhi, Shuang,Liu, Changying,Xu, Fengxiang,Zhao, Aichun,Wang, Xiling,Tang, Xing,Yu, Maode,Li, Zhengang,Huang, Ping.

[4]A Comparative Transcriptome Analysis Leads to New Insights into the Molecular Events Governing Root Formation in Mulberry Softwood Cuttings. Du, Wei,Ban, Yueyuan,Nie, Hao,Tang, Zhuang,Du, Xiaolong,Cheng, Jialing,Du, Wei.

[5]Characterization of Stilbene Synthase Genes in Mulberry (Morus atropurpurea) and Metabolic Engineering for the Production of Resveratrol in Escherichia coli. Wang, Chuanhong,Zhi, Shuang,Liu, Changying,Xu, Fengxiang,Zhao, Aichun,Wang, Xiling,Ren, Yanhong,Yu, Maode,Li, Zhengang.

[6]Acaricide resistance of Tetranychus cinnabarinus (Acari: Tetraychidae) from mulberry plantations in southwest China. Luo, Yan-Jie,Ding, Wei,Luo, Yan-Jie,Ni, Jing,Liu, Yong-Guang,Jiang, Xiu-Jun,Chai, Jian-Ping,Xie, Dao-Yan,Da, Ai-si,Huang, Ping,Li, Shao-xiang. 2013

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：云南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台