Determination of Trigonelline, Nicotinic Acid, and Caffeine in Yunnan Arabica Coffee by Microwave-Assisted Extraction and HPLC with Two Columns in Series
文献类型： 外文期刊
作者： Liu, Hongcheng 1 ; Shao, Jinliang 1 ; Li, Qiwan 1 ; Li, Yangang 1 ; Yan, Hong Mei 1 ; He, Lizhong 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Inst Qual Standard & Testing Technol, Minist Agr Kunming, Supervis & Testing Ctr Farm Prod Qual, Kunming 650223, Peoples R China
期刊名称：JOURNAL OF AOAC INTERNATIONAL （ 影响因子：1.913； 五年影响因子：1.754 ）
ISSN： 1060-3271
年卷期： 2012 年 95 卷 4 期
页码：
收录情况： SCI
摘要： A simple, rapid method was developed for simultaneous extraction of trigonelline, nicotinic acid, and caffeine from coffee, and separation by two chromatographic columns in series. The trigonelline, nicotinic acid, and caffeine were extracted with microwave-assisted extraction (MAE). The optimal conditions selected were 3 min, 200 psi, and 120 C. The chromatographic separation was performed with two columns in series, polyaromatic hydrocarbon C-18(250 x 4.6 mm id, 5 pm particle size) and Bondapak NH2(300 x 3.9 mm id, 5 pm particle size). lsocratic elution was with 0.02 M phosphoric acid methanol (70 + 30, v/v) mobile phase at a flow rate of 0.8 mt./min. Good recoveries and RSD values were found for all analytes in the matrix. The LOD of the three compounds was 0.02 mg/L, and the LOQ was 0.005% in the matrix. The concentrations of trigonelline, nicotinic acid, and caffeine in instant coffee, roasted coffee, and raw coffee (Yunnan Arabica coffee) were assessed by MAE and hot water extraction; the correlation coefficients between concentrations of the three compounds obtained were close to 1.
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