Establishment of a CRISPR-based system for rapidly detecting the target-site resistance of American sloughgrass (Beckmannia syzigachne) to Pinoxaden
文献类型： 外文期刊
作者： Ban, Yaxin 1 ; Zhang, Zhanzhan 1 ; Wei, Jianguo 1 ; Xu, Gaofeng 2 ; Ma, Youmei 1 ; Yin, Shaojing 1 ; Dong, Liyao 1 ; Feng, Zhike 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 211800, Peoples R China
2.Yunnan Acad Agr Sci, Inst Agr Environm & Resources Res, Kunming, Peoples R China
关键词：
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：3.8； 五年影响因子：4.3 ）
ISSN： 1526-498X
年卷期： 2025 年 81 卷 9 期
页码：
收录情况： SCI
摘要： BACKGROUNDWeeds resistant to herbicides pose significant challenges in crop production, making early resistance monitoring crucial for timely control of resistant weeds. Prolonged use of ACCase-inhibiting herbicides, like pinoxaden, has led to the evolution of high-level resistance in weed populations over time. American sloughgrass (Beckmannia syzigachne), is a noxious grass weed, that severely impacts the yield and quality of wheat and rapeseed crops.RESULTSTo accurately and rapidly detect the mutations in the target gene of B. syzigachne, we developed a novel rapid detection method based on the CRISPR-Cas12b/sgRNA system to evaluate the target mutation at amino acid position 1781 of the ACCase gene. By optimizing various reaction conditions, the novel detection system can assess target-site resistance of B. syzigachne to pinoxaden within 40 min at a constant temperature of 54 degrees C. This novel system exhibits excellent specificity, high sensitivity, simplicity in procedure, also with time-efficient and high throughput.CONCLUSIONThis study presents an efficient method based on the CRISPR-Cas12b system for rapidly detecting the target-site resistance, which will facilitate the precise management of resistant weeds. (c) 2025 Society of Chemical Industry.
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