Two novel dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides identified from truffle (Tuber sinense) by peptidomics, in silico, and molecular docking analysis
文献类型： 外文期刊
作者： Li, Yujing 1 ; Zhang, Fei 1 ; Gong, Jiashun 1 ; Peng, Chunxiu 3 ;
作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Agroprod Proc Res Inst, Kunming 650221, Yunnan, Peoples R China
3.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China
关键词： Tuber sinense; DPP -IV inhibitory peptide; in silico screening; molecular docking; in vivo hypoglycemic effect
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.3； 五年影响因子：4.6 ）
ISSN： 0889-1575
年卷期： 2023 年 121 卷
页码：
收录情况： SCI
摘要： The aim of present study is to explore the endogenous dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides from the edible truffle species Tuber sinense. The 4038 endogenous peptides from fresh ascocarps were identified using a peptidomics analysis based on the ultra-performance liquid chromatography-quadrupole-Orbitrap-tandem mass spectrometry (UPLC-Q-Orbitrap-MS2). Afterward, ten DPP-IV inhibitory peptides were screened through Peptide Ranker software and BIOPEP database. According to the absorption, distribution, metabolism, excretion, toxicities (ADMET) analysis and molecular docking analysis, two peptides (APIKIPVGPGTL and IEDP-DAPPPPPPREVIKN) were finally confirmed. The affinity values of APIKIPVGPGTL and IEDPDAPPPPPPREVIKN were -8.8 and -8.4 Kcal/mol, respectively. Two peptides had hydrogen bonds and hydrophobic interaction with amino acid residue in the S1 and S2 pockets of DPP-IV enzyme. Furthermore, the half-maximal inhibitory concentrations of two peptides were 643.14 +/- 1.79 mu M and 668.29 +/- 1.31 mu M, respectively. The oral glucose tolerance test (OGTT) of two peptides showed excellent hypoglycemic effect at 30 min after gavage. Our work may have important theoretical significance and practical value for further exploring the endogenous peptides of truffle species.
- 相关文献
作者其他论文 更多>>
-
Theabrownin ameliorated lipid accumulation via modulating circadian rhythms in oleic acid-induced HepG2 cells
作者：Zhao, Chunyan;Zhao, Lei;Li, Zelin;Lei, Shuwen;Shan, Bo;Wang, Qiuping;Tan, Chao;Gong, Jiashun;Li, Zelin;Gong, Jiashun;Peng, Chunxiu;Miao, Yue
关键词：Pu-erh tea; Theabrownin; Circadian rhythms; Lipid accumulation; Proteomics; siRNA; HepG2 cells
-
Theabrownin from Pu-erh Tea Improves DSS-Induced Colitis via Restoring Gut Homeostasis and Inhibiting TLR2&4 Signaling Pathway
作者：Zhao, Lei;Zhao, Chunyan;Miao, Yue;Lei, Shuwen;Li, Yujing;Gong, Jiashun;Zhao, Lei;Miao, Yue;Gong, Jiashun;Peng, Chunxiu
关键词：Ulcerative colitis; Intestinal barrier; Differentiation of CD4+T cells; Intestinal microbiota
-
Caffeine regulates both osteoclast and osteoblast differentiation via the AKT, NF-κB, and MAPK pathways
作者：Miao, Yue;Lei, Shuwen;Zhao, Chunyan;Wang, Qiuping;Tan, Chao;Gong, Jiashun;Miao, Yue;Zhao, Lei;Peng, Chunxiu;Gong, Jiashun
关键词：caffeine; osteoclasts; osteoblasts; bone metabolism; ovariectomy
-
Arsenic source analysis of rice from different growing environments and health risk assessment in Hunan Province, China
作者：Liu, Yanfang;Gong, Jiashun;Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Wang, Xinrui;Tong, Litao;Wang, Fengzhong;Fan, Bei;Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Wang, Xinrui;Tong, Litao;Wang, Fengzhong;Fan, Bei;Gong, Jiashun
关键词：Rice; Inorganic arsenic; Health risk assessment; ICP-MS; HPLC-HG-AFS; Irrigation water; Soil; Hunan Province
-
Pangenome analysis provides insight into the evolution of the orange subfamily and a key gene for citric acid accumulation in citrus fruits
作者：Huang, Yue;He, Jiaxian;Xu, Yuantao;Zheng, Weikang;Jiang, Xiaolin;Liu, Zishuang;Wang, Lun;Wang, Xia;Liu, Shengjun;Lu, Zhihao;Liu, Ziang;Yu, Huiwen;Xie, Zongzhou;Li, Chunlong;Ma, Zhaocheng;Jiao, Wenbiao;Zhang, Fei;Larkin, Robert M.;Deng, Xiuxin;Xu, Qiang;Huang, Yue;Xu, Yuantao;Xu, Qiang;Wang, Shaohua;Yue, Jianqiang;Gao, Junyan;Zhou, Xianyan;Long, Chunrui;Chen, Peng;Zeng, Bin;Yang, Shuizhi;Zeng, Xiuli;Guo, Yong-Jie;Guo, Yong-Jie;Zhang, Wen-Fu;Krueger, Robert R.;Smith, Malcolm W.;Ming, Ray
关键词：
-
Effect of different agricultural conditions, practices, and processing on levels of total arsenic and species in cereals and vegetables: A review
作者：Liu, Yanfang;Huang, Yatao;Li, Lin;Xiong, Yangyang;Tong, Litao;Wang, Fengzhong;Fan, Bei;Gong, Jiashun;Gong, Jiashun
关键词：Arsenic; Species; Arsenic concentration; Processing methods; Cooking methods; Agricultural products
-
Theabrownin Isolated from Pu-Erh Tea Enhances the Innate Immune and Anti-Inflammatory Effects of RAW264.7 Macrophages via the TLR2/4-Mediated Signaling Pathway
作者：Zhao, Lei;Miao, Yue;Shan, Bo;Zhao, Chunyan;Gong, Jiashun;Zhao, Lei;Peng, Chunxiu;Gong, Jiashun
关键词：theabrownin; RAW264.7 macrophages; innate immune enhancement; anti-inflammatory effect; TLR2/4; NF-kappa B/MAPK/PI3K-AKT signaling pathways
