Two novel dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides identified from truffle (Tuber sinense) by peptidomics, in silico, and molecular docking analysis
文献类型： 外文期刊
作者： Li, Yujing 1 ; Zhang, Fei 1 ; Gong, Jiashun 1 ; Peng, Chunxiu 3 ;
作者机构： 1.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China
2.Yunnan Acad Agr Sci, Agroprod Proc Res Inst, Kunming 650221, Yunnan, Peoples R China
3.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650201, Yunnan, Peoples R China
关键词： Tuber sinense; DPP -IV inhibitory peptide; in silico screening; molecular docking; in vivo hypoglycemic effect
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.3； 五年影响因子：4.6 ）
ISSN： 0889-1575
年卷期： 2023 年 121 卷
页码：
收录情况： SCI
摘要： The aim of present study is to explore the endogenous dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides from the edible truffle species Tuber sinense. The 4038 endogenous peptides from fresh ascocarps were identified using a peptidomics analysis based on the ultra-performance liquid chromatography-quadrupole-Orbitrap-tandem mass spectrometry (UPLC-Q-Orbitrap-MS2). Afterward, ten DPP-IV inhibitory peptides were screened through Peptide Ranker software and BIOPEP database. According to the absorption, distribution, metabolism, excretion, toxicities (ADMET) analysis and molecular docking analysis, two peptides (APIKIPVGPGTL and IEDP-DAPPPPPPREVIKN) were finally confirmed. The affinity values of APIKIPVGPGTL and IEDPDAPPPPPPREVIKN were -8.8 and -8.4 Kcal/mol, respectively. Two peptides had hydrogen bonds and hydrophobic interaction with amino acid residue in the S1 and S2 pockets of DPP-IV enzyme. Furthermore, the half-maximal inhibitory concentrations of two peptides were 643.14 +/- 1.79 mu M and 668.29 +/- 1.31 mu M, respectively. The oral glucose tolerance test (OGTT) of two peptides showed excellent hypoglycemic effect at 30 min after gavage. Our work may have important theoretical significance and practical value for further exploring the endogenous peptides of truffle species.
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