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The ethanol extract of Garcinia subelliptica Merr. induces autophagy

文献类型： 外文期刊

作者： Ha Kim, Kyun 1 ; Lee, Ji Yeon 1 ; Li, Wan Yi 2 ; Lee, Sangwoo 3 ; Jeong, Han-Sol 1 ; Choi, Jun-Yong 4 ; Joo, Myungsoo 1 ;

作者机构： 1.Pusan Natl Univ, Sch Korean Med, Yangsan 50612, South Korea

2.Yunnan Acad Agr Sci, Inst Med Plants, Kunming 650224, Yunnan, Peoples R China

3.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea

4.Pusan Natl Univ, Korean Med Hosp, Pulm Med Ctr, Lung Canc Clin, Yangsan 50612, South Korea

关键词： Garcinia subelliptica Merr; Human lung carcinoma cells; mTOR; AMPK; Autophagy

期刊名称：BMC COMPLEMENTARY MEDICINE AND THERAPIES

ISSN：

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Garcinia subelliptica Merr. is a multipurpose coastal tree, the potential medicinal effects of which have been studied, including cancer suppression. Here, we present evidence that the ethanol extract of G. subelliptica Merr. (eGSM) induces autophagy in human lung adenocarcinoma cells. Methods Two different human lung adenocarcinoma cell lines, A549 and SNU2292, were treated with varying amounts of eGSM. Cytotoxicity elicited by eGSM was assessed by MTT assay and PARP degradation. Autophagy in A549 and SNU2292 was determined by western blotting for AMPK, mTOR, ULK1, and LC3. Genetic deletion of AMPK alpha in HEK293 cells was carried out by CRISPR. Results eGSM elicited cytotoxicity, but not apoptosis, in A549 and SNU2292 cells. eGSM increased LC3-II production in both A549 and, more extensively, SNU2292, suggesting that eGSM induces autophagy. In A549, eGSM activated AMPK, an essential autophagy activator, but not suppressed mTOR, an autophagy blocker, suggesting that eGSM induces autophagy by primarily activating the AMPK pathway in A549. By contrast, eGSM suppressed mTOR activity without activating AMPK in SNU2292, suggesting that eGSM induces autophagy by mainly suppressing mTOR in SNU2292. In HEK293 cells lacking AMPK alpha expression, eGSM increased LC3-II production, confirming that the autophagy induced by eGSM can occur without the AMPK pathway. Conclusion Our findings suggest that eGSM induces autophagy by activating AMPK or suppressing mTOR pathways, depending on cell types.

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